Taproot

Abstract: Many studies focus on the effects of vegetation cover on water erosion rates, whereas little attention has been paid to the effects of the below ground biomass. Recent research indicates that roots can reduce concentrated flow erosion rates significantly. In order to predict this root effect more accurately, this experimental study aims at gaining more insight into the importance of root architecture, soil and flow characteristics to the erosion-reducing potential of roots during concentrated flow. Treatments were (1) bare, (2) grass (representing a fine-branched root system), (3) carrots (representing a tap root system) and (4) carrots and fine-branched weeds (representing both tap and fine-branched roots). The soil types tested were a sandy loam and a silt loam. For each treatment, root density, root length density and mean root diameter (D) were assessed. Relative soil detachment rates and mean bottom flow shear stress were calculated. The results indicate that tap roots reduce the erosion rates to a lesser extent compared with fine-branched roots. Different relationships linking relative soil detachment rate with root density could be established for different root diameter classes. Carrots with very fine roots (D < 5 mm) show a similar negative exponential relationship between root density and relative soil detachment rate to grass roots. With increasing root diameter (5 < D < 15 mm) the erosion-reducing effect of carrot type roots becomes less pronounced. Additionally, an equation estimating the erosion-reducing potential of root systems containing both tap roots and fine-branched roots could be established. Moreover, the erosion-reducing potential of grass roots is less pronounced for a sandy loam soil compared with a silt loam soil and a larger erosion-reducing potential for both grass and carrot roots was found for initially wet soils. For carrots grown on a sandy loam soil, the erosion-reducing effect of roots decreases with increasing flow shear stress. For grasses, grown on both soil types, no significant differences could be found according to flow shear stress. The erosion-reducing effect of roots during concentrated flow is much more pronounced than suggested in previous studies dealing with interrill and rill erosion. Root density and root diameter explain the observed erosion rates during concentrated flow well for the different soil types tested. Copyright © 2007 John Wiley & Sons, Ltd.

Abstract: Aluminum (Al) toxicity and phosphorus (P) deficiency often coexist in acid soils that severely limit crop growth and production, including soybean (Glycine max). Understanding the physiological mechanisms relating to plant Al and P interactions should help facilitate the development of more Al-tolerant and/or P-efficient crops. In this study, both homogeneous and heterogeneous nutrient solution experiments were conducted to study the effects of Al and P interactions on soybean root growth and root organic acid exudation. In the homogenous solution experiments with a uniform Al and P distribution in the bulk solution, P addition significantly increased Al tolerance in four soybean genotypes differing in P efficiency. The two P-efficient genotypes appeared to be more Al tolerant than the two P-inefficient genotypes under these high-P conditions. Analysis of root exudates indicated Al toxicity induced citrate exudation, P deficiency triggered oxalate exudation, and malate release was induced by both treatments. To more closely mimic low-P acid soils where P deficiency and Al toxicity are often much greater in the lower soil horizons, a divided root chamber/nutrient solution approach was employed to impose elevated P conditions in the simulated upper soil horizon, and Al toxicity/P deficiency in the lower horizon. Under these conditions, we found that the two P-efficient genotypes were more Al tolerant during the early stages of the experiment than the P-inefficient lines. Although the same three organic acids were exuded by roots in the divided chamber experiments, their exudation patterns were different from those in the homogeneous solution system. The two P-efficient genotypes secreted more malate from the taproot tip, suggesting that improved P nutrition may enhance exudation of organic acids in the root regions dealing with the greatest Al toxicity, thus enhancing Al tolerance. These findings demonstrate that P efficiency may play a role in Al tolerance in soybean. Phosphorus-efficient genotypes may be able to enhance Al tolerance not only through direct Al-P interactions but also through indirect interactions associated with stimulated exudation of different Al-chelating organic acids in specific roots and root regions.

Abstract: Symbiotic mycorrhizal fungi play an important role in the absorption of soil nutrients and water by most plants. It has been suggested that hydraulically lifted water might maintain the integrity of the external mycorrhizal mycelium during drought. We tested this hypothesis in the obligately mycorrhizal species, coast live oak (Quercus agrifolia), using a microcosm system that separated the effects of hydraulic lift in roots from those in the external mycelium. Mycorrhizal oak seedlings were established in microcosms comprising three discrete compartments for (1) upper roots, (2) tap roots, and (3) external fungal mycelium. Eight months after planting, a drought treatment was initiated: irrigation to the upper root and fungal chambers was terminated and only irrigation to the taproot compartment was maintained. After 3, 12, 30, 50, 70 and 80 days of drought, tracers were injected into the taproot compartment at dusk. At dawn the following morning, mycorrhizal hyphae (EM and AM) and spores (AM) in upper root and fungal compartments were extensively labeled with the tracers. In contrast, no labeling was observed when tracers were injected into the taproot compartment during daytime. Nocturnal water translocation from plant to mycorrhizal fungi occurred in association with hydraulic lift. Saprotrophic/parasitic fungi in the microcosms were not labeled, suggesting a direct water transfer from plants to their mycorrhizal mutualists and not to other fungi in the soil. Even after prolonged drought (70-80 days), mycorrhizal hyphae persisted in soils with water potential values as low as -20 MPa. Maintaining mycorrhizal activity through direct water translocation could potentially improve the nutrient status of deep-rooted plants during periods when the fertile upper soil is dry.