About: TAF1 is a research topic. Over the lifetime, 490 publications have been published within this topic receiving 55036 citations. The topic is also known as: BA2R & CCG1.
TL;DR: A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Abstract: We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
TL;DR: Experiments reveal crosstalk between histone modifications and the transcription factor TFIID, which has important implications for regulation of RNA polymerase II-mediated transcription in higher eukaryotes.
TL;DR: Results suggest that one or more of the TAF polypeptides confer coactivator function in NTF-1, a developmentally regulated transcription factor in Drosophila.
TL;DR: It is found that while TFIID and SAGA make overlapping contributions to the expression of all genes, TFIid function predominates at approximately 90% and S AGA at approximately 10% of the measurable genome.