Topic
Superose
About: Superose is a research topic. Over the lifetime, 102 publications have been published within this topic receiving 2804 citations.
Papers published on a yearly basis
Papers
TL;DR: A process incorporating aqueous two-phase extraction, hydrophobic interaction chromatography (HIC) and size-exclusion chromatography for the purification of human immunoglobulin G (IgG) from a Chinese hamster ovary (CHO) cell supernatant is evaluated.
103 citations
TL;DR: The adsorption characteristics of a variety of synthetic peptide hormones and di-, tri- and tetrapeptides on Cu(II) immobilized on two commercially available high-performance chelating gels run under various experimental conditions suggest that peptides containing aromatic and hydroxy amino acids are retarded more than those which lack them.
98 citations
TL;DR: Plasma lipoprotein fractions from inbred C57BL/6J mice and outbred ICR mice were prepared by sequential density ultracentrifugation using density ranges that were optimized for separating mouse lipoproteins, or by Superose 6 HR10/30 fast performance liquid chromatography (FPLC).
82 citations
TL;DR: In this paper, a recombinant F1 antigen (rF1) was purified by ammonium sulfate fractionation followed by FPLC Superose gel filtration chromatography.
Abstract: Yersinia pestis, the causative organism of plague, produces a capsular protein (fraction 1 or F1 antigen) that is one of the major virulence factors of the bacterium. We report here the production, structural and immunological characterisation of a recombinant F1 antigen (rF1). The rF1 was purified by ammonium sulfate fractionation followed by FPLC Superose gel filtration chromatography. Using FPLC gel filtration chromatography and capillary electrophoresis, we have demonstrated that rF1 antigen exists as a multimer of high molecular mass. This multimer dissociates after heating in the presence of SDS and reassociation occurs upon the removal of SDS. Using circular dichroism, we have monitored the reassociation of monomeric rF1 into a multimeric form. Mice immunised with monomeric or multimeric rF1 develop similar immune responses, but mice immunised with monomeric rF1 were significantly less well protected against a challenge of 1×106 cfu of Y. pestis than mice immunised with multimeric rF1 (1/7 compared with 5/7). The significance of this result in terms of the structure and the function of rF1 is discussed.
68 citations
TL;DR: A method to isolate a protein related to the diacylglycerol:acyltransferase (DGAT) activity in rat liver microsomes has been developed and a 60 kDa protein was highly enriched in the DGAT-containing retained fraction from the immunoaffinity chromatography.
65 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2010 | 2 |
| 2009 | 1 |
| 2008 | 1 |
| 2007 | 2 |
| 2005 | 2 |
| 2004 | 1 |