TL;DR: Red spinach flour doses as feed on the quality of the Sumatra barb showed that supplementation of red spinach flour by 6% with carotenoid could increase sumatra barb color qualities.
Abstract: The Sumatra barb (Puntigrus tetrazona) is one of the Indonesia indigenous ornamental fish from Sumatra island. The breeding of sumatra barb fish has actually seen a decline in color quality because this species cannot produce carotenoid in the body. To solve this problem, needed an alternative using supplementation the carotenoid in the feed. The objective of this study was aimed to determine the effectiveness of red spinach flour doses as feed on the quality of the sumatra barb. The sumatra barb ornamental fish, with weight about 0.54 g and length about 3.50 cm, were reared in the aquarium (30 cm x 20 cm x 20 cm). The completely randomized design with 4 treatments (A=0% (control), B=2%, C=4%, and D=6%) and 3 replications were analyzed in this study. Parameters in this study consisted of body skin color qualities (e.g. L = lightness, C = chroma, and H = hue) and the number of carotenoid in the skin, fins, and meat. The fish were reared for 42 days and given feed 3 times per day. This result showed that supplementation of red spinach flour by 6% with carotenoid 45.26 ppm in feed, 50.44 ppm in fins, 16.10 ppm in the skin, and 3.69 ppm in meat, could increase sumatra barb color qualities. It was indicated decreasing lightness 60.83%, increasing chroma 20.57%, and sustaining hue 87,09°.AbstrakIkan sumatra albino (Puntigrus tetrazona) merupakan salah satu ikan hias asli Indonesia yang berasal dari pulau Sumatera. Budidaya ikan sumatra albino mengalami penurunan dalam kualitas warna karena spesies ini tidak dapat memproduksi karotenoid dalam tubuhnya. Guna mengatasi permasalahan ini, maka dibutuhkan sebuah alternatif berupa penambahan karotenoid dalam pakan. Penelitian ini bertujuan untuk mengkaji efektivitas dosis tepung bayam merah sebagai pakan terhadap kualitas warna ikan sumatra albino. Ikan hias sumatra albino dengan bobot 0,54 g dan panjang 3,50 cm dipelihara di dalam akuarium (30 cm x 20 cm x 20 cm).Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empatperlakuan (A=0% (kontrol), B=2%, C=4%, dan D=6%) dan tiga ulangan. Parameter yang diamati meliputi nilai kualitas warna kulit ikan (L=lightness, C=chroma, dan H=hue) dan total karotenoid pada kulit, sirip,serta daging ikan sumatra albino. Ikan dipelihara selama 42 hari dan diberi pakan tigakali sehari. Hasil penelitian menunjukkan bahwapenambahantepung bayam merah pada dosis 6%dengan kandungan karotenoid 45,26 ppm pada pakan, 50,44 ppm pada sirip, 16,10 ppm pada kulit, dan 3,69 ppm pada daging, dapat meningkatkan kualitas warna ikan sumatra albino. Hal tersebut diindikasikan dengan menurunnya nilai L=60,83%, meningkatnya nilai C=20,57%, dan mempertahankan nilai H=87,09°.
TL;DR: The Sumatra barb Barbus tetrazona exhibits two trunk banding patterns: a four- banded and a five-banded pattern characteristic of B. t.
Abstract: The Sumatra barb Barbus tetrazona exhibits two trunk banding patterns: a four-banded and a five-banded pattern characteristic of B. t. tetrazona and B. t. partipentazona, respectively. Segregation patterns in the progenies from 11 different matings suggest monogenic inheritance of trunk banding in this species.
TL;DR: The trans formation of gonads from ovarian to testicular nature occurring in future males seemed to be completed rapidly, since no ovarian characteristics remained in the testes of the fish examined later than 60 days after hatching, though residual ovarian cavities were occasionally retained in the Testis of adult males.
Abstract: The process of sex differentiation and development of the gonad in the Sumatra barb, Barbus tetrazona tetrazona, reared in laboratory conditions was studied histologically in the present study. Gonads of all juvenile fish were initially provided with ovarian structures, which were characterized by the development of germ cells into meiotic prophase and auxocyte stages and by the formation of ovarian cavity, during the period from 20 to 25 days after hatching. The ovary continued to grow steadily in part of the fish on successive days, whereas in other fish it remained in ill-developed states in which sparsely distributed oocytes, a relatively large number of gonial germ cells and compact stromal tissue with increased somatic cells were notable. In gonads of the latter type, testicular development began with clustering of gonial germ cells to form primordial seminal lobules, which indicated definitely the differentiation of spermatogonia, and subsequent initiation of spermatogenesis in some of them. Concomitant degenerative changes of oocytes accompanied in some cases by marked infiltration of somatic cells over the stromal tissue were also prominent in these gonads. Intersexual gonads with developing male germ cells and degenerating oocytes thus occurred apparently within 45 and 50 days after hatching. The trans formation of gonads from ovarian to testicular nature occurring in future males seemed to be completed rapidly, since no ovarian characteristics remained in the testes of the fish examined later than 60 days after hatching, though residual ovarian cavities were occasionally retained in the testis of adult males. Thus true gonochorism with a nearly 1:1 sex ratio was established following the intersexual stage in the Sumatra barb.
TL;DR: The experiments proved the hypothesis that the visible patterns on Sumatra Barb (Puntigrus tetrazona) body can be used for individual identification and substituted current practice of fish identification based on tagging and marking.
Abstract: The paper deal with the individual fish identification of the same species based on digital image of the fish. The proof of concept of image based individual identification is introduced on the small group fish. The method is completely noninvasive and can overcome the disadvantages of standard invasive identification such as tagging. The experiments proved the hypothesis that the visible patterns on Sumatra Barb (Puntigrus tetrazona) body can be used for individual identification. In the first step, the database of 43 fish (was created by the taking of the images of fish in different pose. Images were taken in an aquarium with a water. After data collection, data was processed by the image processing methods to determine the features. The simple nearest neighbor classification was used to test individual identification. The accuracy of classification was 100%. The method proved the hypothesis that the visible pattern on Sumatra Barb can be used for fully automated individual fish identification. It can be substituted current practice of fish identification based on tagging and marking. The long-term stability of the pattern and the classification power for large fish group should be studied in the future.
TL;DR: This study shows that visible patterns and image processing methods can be used to automatically identify individual fish of the same species and should work for any fish with unique visible skin patterns, for example, for commercial fish species like Atlantic salmon and European perch.
Abstract: Non-invasive fish identification of individuals can provide new possibilities for the monitoring of fish cultivation, improve and make fish production technologies less demanding for farmers, and increase fish welfare. The aim of this research is to confirm the idea of automatic non-invasive image-based fish identification of individuals using visible features on a fish body and prove the pattern stability during the fish cultivation period. Visible patterns, such as black stripes along the body of a Sumatra barb (Puntigrus tetrazona), were used for machine identification of individual fish. Two experiments were completed: a short-term experiment (43 fish) to show the uniqueness of the stripe patterns for identification, and a long-term experiment (25 fish) to test the stability of patterns during the cultivation period. The overall accuracy of classification was 100% for data collection in one day and 88% between two data collection times. This study shows that visible patterns and image processing methods can be used to automatically identify individual fish of the same species. This is not just limited to Sumatra barb—the concept should work for any fish with unique visible skin patterns, for example, for commercial fish species like Atlantic salmon (Salmo salar) and European perch (Perca fluviatilis).