TL;DR: The new genus Stygiolobus is described, which comprises three isolates of extremely thermophilic chemolithoautotrophic archaebacteria obtained from solfataric fields on ao Miguel Island, Azores and are the first strictly anaerobic members of the order Sulfolobales.
Abstract: On the basis of three isolates (strains FC6T [T = type strain], FC4, and RG1) of extremely thermophilic chemolithoautotrophic archaebacteria obtained from solfataric fields on ao Miguel Island, Azores, the new genus Stygiolobus is described These isolates grow obligately chemolithotrophically by reduction of S0 with H2 (H2-S0 lithotrophy) and are the first strictly anaerobic members of the order Sulfolobales With a DNA G+C content of 38 mol%, the Stygiolobus isolates resemble Sulfolobus spp, which, however, are faculatively organotrophic and aerobic S0 oxidizers The new isolates are also distinct from Acidianus spp, which resemble Stygiolobus by growing by H2-S0 lithotrophy However, Acidianus spp can also grow aerobically by S0 oxidation and have G+C contents of 31 mol% At this time, only one species of the genus Stygiolobus is known, Stygiolobus azoricus sp nov; the type strain of S azoricus is strain FC6 (= DSM 6296)
TL;DR: Three spherical thermoacidophilic archaea obtained from acidic hot springs located in Ohwaku Valley, Hakone, Japan are described as members of the new genus Sulfurisphaera, and the type strain is TA-1T (= IFO 15161T).
Abstract: Three spherical thermoacidophilic archaea (strains TA-1T, TA–13, TA–14) were obtained from acidic hot springs located in Ohwaku Valley, Hakone, Japan. All the isolates are facultatively anaerobic, and grew optimally at around 85 °C pH 2·0. Isolate TA-1Twas characterized further. The G+C content of DNA from TA-1T is 33 mol%. Although these properties resemble those of the genus Acidianus, the sequence of the 165 rRNA gene from strain TA-1Twas more similar to that of species of Stygiolobus than of Acidianus. DNA-DNA hybridization experiments also indicated that strain TA-1Tis clearly distinguished phylogenetically from the members of Acidianus, Sulfolobus and Metallosphaera. On the basis of the distinct physiological and molecular properties, we describe the new strains as members of the new genus Sulfurisphaera. The type species of the genus is Sulfurisphaera ohwakuensis, and the type strain of the species is TA-1T(= IFO 151 SI1).