TL;DR: Although some generic relationships are not highly resolved, the analyses provide strong support for Zigadenus glaberrimus as sister to the rest of the tribe, and Amianthium muscitoxicum as closely related to Veratrum s.l.
Abstract: The circumscription and relationships of genera within the tribe Melanthieae (29 representative taxa) were evaluated using parsimony analyses of ITS (nuclear ribosomal) and trnL-F (plastid) DNA sequence data, alone and in combination. Proposed new generic circumscriptions, strongly supported by the tree statistics and topologies in all analyses, are correlated with potential morphological synapomorphies at the proper level of universality. Based on the molecular cladograms, Stenanthium is biphyletic, and the traditional Zigadenus s.1. (sensu lato) is polyphyletic. Amianthium and Schoenocaulon are distinct entities; the Veratrum complex is conservatively treated as one large monophyletic genus (including Melanthium). Although some generic relationships are not highly resolved, the analyses provide strong support for Zigadenus glaberrimus as sister to the rest of the tribe, and Amianthium muscitoxicum as closely related to Veratrum s.l. As a result of these analyses, seven genera (some with novel circumscription) are recognized within the tribe Melanthieae: Amianthium, Anticlea, Schoenocaulon, Stenanthium, Toxicoscordion, Veratrum, and Zigadenus.
TL;DR: The molecular and morphological data support generic recircumscription in Melanthieae and also validate several generalizations concernine character evolution within the tribe, as well as among the tribes of the family.
Abstract: Melanthiaceae s.l. comprises five tribes: Chionographideae, Heloniadeae, Melanthieae, Parideae, and Xerophylleae-each defined by distinctive autapomorphies. The most morphologically diverse tribe Melanthieae, now with seven genera, had not been subject to rigorous phylogenetic character study prior to the current series of investigations that also include an overview of the family. Data from our publications and studies underway are here assessed and integrated, providing a useful overview of Melanthiaceae, and especially of Melanthieae. The results of parsimony analyses of ITS (nuclear ribosomal) and trnL-F (plastid) DNA sequence data correlate with potentially synapomorphic phenotypic characters for genera of Melanthieae, including habit form, rootstock type, bulb shape, inflorescence structure, indumentum type, tepal shape, nectary morphology, and ovary position. Sequence data also correlate well with the pattern of variation in chromosome number. The molecular and morphological data support generic recircumscription in Melanthieae and also validate several generalizations concernine character evolution within the tribe, as well as among the tribes of the family.
TL;DR: Reinstatement of segregate genera and the accompanying recombinations for species in the former polyphyletic Zigadenus complex allow for a revised, practical taxonomy that defines monophyletic groups and reflects phylogenetic relationships within tribe Melanthieae (Melanthiaceae).
Abstract: Reinstatement of segregate genera and the accompanying recombinations for species in the former polyphyletic Zigadenus complex allow for a revised, practical taxonomy that defines monophyletic groups and reflects phylogenetic relationships within tribe Melanthieae (Melanthiaceae). Nine new species combinations result from recircumscription of Zigadenus Michaux and Stenanthium (A. Gray) Kunth and from recognition of Anticlea Kunth and Toxicoscordion Rydberg. The genus Stenanthella Rydberg and the basionyms for Stenanthium leimanthoides (A. Gray) Zomlefer & Judd (Amianthium leimanthoides A. Gray) and Anticlea sachalinensis (F. Schmidt) Zomlefer & Judd (Stenanthium sachalinense F. Schmidt) are lectotypified.
TL;DR: The genus Stenanthium, distributed through much of eastern United States, has previously been circumscribed with four species as discussed by the authors, S. macrum, S. leimanthoides (Melanthiaceae), S. tennesseense, and S. densum.
Abstract: The genus Stenanthium, distributed through much of eastern United States, has previously been circumscribed with four species. Herbarium and field research on Stenanthium densum and S. leimanthoides (Melanthiaceae) support the recognition of two new segregate species, S. macrum and S. tennesseense. Data are presented on morphology, phenology, habitat, and distribution of each of these four species. Selected specimen records, distribution maps, field photographs, and a key to these four species are provided.