Topic
Staphylococcus microti
About: Staphylococcus microti is a research topic. Over the lifetime, 4 publications have been published within this topic receiving 51 citations.
Papers
TL;DR: Two strains of Gram-positive cocci were isolated from viscera of common voles with generalized Brucella microti infection in the Czech Republic and biochemical features and phylogenetic analysis showed that the strains are representatives of the genus Staphylococcus and assigned Staphyllococcus muscae as the nearest relative.
Abstract: Two strains of Gram-positive cocci were isolated from viscera
of common voles (Microtus arvalis Pallas) with generalized
Brucella microti infection in the Czech Republic. Biochemical
features and phylogenetic analysis based on 16S rRNA gene
sequences showed that the strains are representatives of the
genus Staphylococcus and assigned Staphylococcus muscae as the
nearest relative. A detailed characterization done by
ribotyping, rpoB and hsp60 gene sequencing, whole-cell protein
analysis and rep-PCR using the (GTG)5 primer differentiated the
two strains from all described staphylococci. DNA-DNA
hybridization with the type strain of S. muscae demonstrated
that the two strains should be considered as members of a novel
species (26.8% reassociation). The two analysed strains were
found to be coagulase-negative, novobiocin-susceptible,
oxidase-negative cultures, phenotypically close to one another,
but showing differences in ribotype profiles. The major fatty
acids were iso-C15 : 0, iso-C17 : 0, anteiso- C15 : 0, C18 :
2v6,9c/anteiso-C18 : 0, C18 : 0 and C18 : 1v9c. MK-7 was the
predominant isoprenoid quinone, with minor amounts of MK-6 and
MK-8. The polar lipid profile was composed of the major lipids
diphosphatidylglycerol and phosphatidylglycerol and several
unknown lipids. These results proved that the two isolates
represent a novel staphylococcal species. The name proposed for
this novel taxon is Staphylococcus microti sp. nov.; the type
strain is 4005-LJ(m)T (5CCM 4903T 5CCUG 55861T 5DSM 22147T).
31 citations
TL;DR: This report is, to the authors' knowledge, the first ever case of bovine mastitis caused by S. microti and the first instance of isolation of this microorganism from domesticated animals.
24 citations
TL;DR: Twenty coagulase-negative Staphylococcus strains displaying alpha-haemolysis on sheep-blood agar were isolated from the noses of different pigs in Switzerland and represent a novel species of the genus Staphlyococcus, for which the name Staphyllococcus rostri sp.
Abstract: Twenty coagulase-negative Staphylococcus strains displaying alpha-haemolysis (delta-haemolysin) on sheep-blood agar were isolated from the noses of different pigs in Switzerland. The strains were Gram-stain-positive, non-motile cocci, catalase-positive and coagulase-negative. Sequence analysis of the 16S rRNA gene, sodA, rpoB, dnaJ and hsp60 and phylogenetic characteristics revealed that the strains showed the closest relatedness to Staphylococcus microti CCM 4903(T) and Staphylococcus muscae DSM 7068(T). The strains can be differentiated from S. microti by the absence of mannose fermentation and arginine arylamidase and from S. muscae by the absence of beta-glucuronidase activity and production of alkaline phosphatase. The chosen type strain ARI 262(T) shared 20.1 and 31.9 % DNA relatedness with S. microti DSM 22147(T) and S. muscae CCM 4903(T), respectively, by DNA-DNA hybridization. iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(17 : 0) were the most common fatty acids. Cell-wall structure analysis revealed the peptidoglycan type A3alpha l-Lys-Gly(2)-l-Ser-Gly (type A11.3). The presence of teichoic acid was determined by sequencing the N-acetyl-beta-d-mannosaminyltransferase gene tarA, which is involved in biosynthesis of ribitol teichoic acid. Menaquinone 7 (MK-7) was the predominant respiratory quinone. The G+C content of ARI 262(T) was 38.8 mol%. The isolated strains represent a novel species of the genus Staphylococcus, for which we propose the name Staphylococcus rostri sp. nov. The type strain is ARI 262(T) (=DSM 21968(T) =CCUG 57266(T)) and strain ARI 602 (=DSM 21969 =CCUG 57267) is a reference strain.
23 citations
TL;DR: Staphylococcus microti DSM 22147 was isolated from viscera of common voles with generalized Brucella microti infection in the Czech Republic and includes a genome of 2,381,859 bp (38.0% GC content) without any plasmids.
Abstract: Staphylococcus microti DSM 22147 was isolated from viscera of common voles (Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. To the best of our knowledge, the genome sequence of the species S. microti has not been previously studied. The complete genome sequence of strain DSM 22147 includes a genome of 2,381,859 bp (38.0% GC content) without any plasmids.
1 citations
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Performance Metrics
| Year | Papers |
|---|---|
| 2018 | 1 |
| 2016 | 1 |
| 2010 | 2 |