Spot analysis

Abstract: An analytical image processing system is used to analyze spots on color electrophoretograms. In one embodiment, the boundary of a spot chosen by the user is uniquely defined using polar cross-sections and is illustrated on a user interactive display. In such manner, the user is provided with visual confirmation of the shape of the spot used as a basis for generating output data relating to the spot analysis. The output data includes information not only about the two-dimensional area of the spot but also the intensity thereof to provide a useful measure of the quantity of protein present in the spot.

Abstract: Sensitive and selective qualitative tests based on chemical reactions using small amounts of complex and reagents are referred to as “spot tests” The target is to reach the utmost sensitivity and selectivity with a minimum of physical and chemical operations Rapid advances in spectral, chromatographic, nuclear, mass spectroscopic and electroanalytical research have changed analytical chemistry spectacularly and developed highly sophisticated analytical tools At the same time another development has taken place in certain distinct areas of application in exactly the opposite direction This is the search for simple, compact and inexpensive analytical devices for semiquantitative evaluation of certain elements or compounds where such approximate evaluation has diagnostic value at least in the first stage of examination Major manufacturers of chemical reagents concentrated their efforts mainly in a number of applicative areas, namely clinical analysis, forensic tests, air and water quality control (QC) tests and soil and food QC tests It must be emphasized that the applicability of spot test methodology is limited and for the exact determination of chemical substances, complex analytical procedures are unavoidable Although the use of spot and screening tests is marginal, the margin, however, is quite significant