TL;DR: The utilization of spermatophores has strong implications on the possibilities of sperm competition and cryptic female choice and may account for the occurrence of a postcopulatory passive phase or prolonged mating in some species.
TL;DR: It is once more confirmed that egg characters provide valuable information for cladistic analysis and identification purposes and a high degree of congruence is observed for the morphological data and a recently assembled molecular data set.
Abstract: The egg morphology of 28 species of Diopsidae is described based on scanning electron microscopy and semi-thin sections for selected species from the following genera: Chaetodiopsis, Cyrtodiopsis, Diasemopsis, Diopsis, Eurydiopsis, Teleopsis, Teloglabrus, Trichodiopsis. Generic descriptions and a key to genera based on egg characters is presented. A data set for phylogenetic analysis is assembled and subjected to the first morphology-based numerical cladistic analysis for the family. The data set consists of 44 adult characters taken from the Diopsidae literature and 10 new egg characters. Cladistic analysis confirms the traditional concept of the family Diopsidae composed of the Centrioncinae (lacking eye stalks) and Diopsinae (with eye stalks) and the Syringogastridae are likely to be the sister group of Diopsidae. Within the Diopsinae, the genus Sphyracephala (including Pseudodiopsis) with its relatively short eye stalks is monophyletic and the sister group of the remaining Diopsinae. The following higher-level phylogenetic hypothesis is proposed (Centrioncinae (Sphyracephala ((Diasemopsis, Trichodiopsis, Chaetodiopsis) ((Teleopsis, Cyrtodiopsis) (Eurydiopsis, Diopsis))))). A high degree of congruence is observed for the morphological data and a recently assembled molecular data set. It is once more confirmed that egg characters provide valuable information for cladistic analysis and identification purposes.
TL;DR: The results are consistent with an association between the microbes and enhanced male fertility and possible explanations for the pattern of distribution and effects on male fertility include a predisposition for acquiring Type A Wolbachia by these flies and accommodation by the host genome to bacterial presence.
Abstract: Distribution and reproductive effectsof Wolbachia in stalk-eyed flies (Diptera: Diopsidae)
TL;DR: The phylogeny of stalk-eyed Xies (Diopsidae) has been triggered by their remarkable lateral elongation of the head into stalks distally carrying the eyes and antennae, making it a useful and popular study system to address a variety of evolutionary questions.
TL;DR: It is demonstrated that chromosome rearrangements often occur in the genus Sphyracephala, the extant genus forming a basal branch of Diopsinae.
Abstract: The eye stalks in Diopsidae (Bilberg, 1820) have been widely examined, but the evolutionary origin of this unique trait remains unclear. Thus, further studies of Sphiracephala (Say, 1828), the extant genus forming a basal branch of Diopsinae, are needed. The present study aimed to identify the karyotype of Sphyracephala detrahens (Walker, 1860) with conventional Giemsa staining. Cytogenetic analysis revealed a diploid number of 2n = 10 including two pairs of metacentric chromosomes, a pair of telocentric chromosomes, a pair of dot-like chromosomes, and a pair of sex chromosomes in S. detrahens. The congener Sphyracephala brevicornis (Say, 1817) has been reported to have the same diploid number, 2n = 10, but different chromosome formula. These results demonstrate that chromosome rearrangements often occur in the genus Sphyracephala.