Topic
Sperm sorting
About: Sperm sorting is a research topic. Over the lifetime, 225 publications have been published within this topic receiving 9032 citations.
Papers published on a yearly basis
Papers
TL;DR: The phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.
Abstract: Intact, viable X and Y chromosome-bearing sperm populations of the rabbit were separated according to DNA content with a flow cytometer/cell sorter. Reanalysis for DNA of an aliquot from each sorted population showed purities of 86% for X-bearing sperm and 81% for Y-bearing sperm populations. Sorted sperm were surgically inseminated into the uterus of rabbits. From does inseminated with sorted X-bearing sperm, 94% of the offspring born were females. From does inseminated with sorted Y-bearing sperm from the same ejaculates, 81% of the offspring were males. The probability of the phenotypic sex ratios differing from 50:50 were p less than 0.0003 for X-sorted sperm and p less than 0.004 for Y-sorted sperm. Thus, the phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.
469 citations
TL;DR: A self-contained integrated microfluidic system that can separate motile sperm from small samples that are difficult to handle using conventional sperm-sorting techniques, and opens the way for convenient bioassays based on sperm motility including at-home motiles sperm tests.
Abstract: This paper describes a self-contained integrated microfluidic system that can separate motile sperm from small samples that are difficult to handle using conventional sperm-sorting techniques. The device isolates motile sperm from nonmotile sperm and other cellular debris, based on the ability of motile sperm to cross streamlines in a laminar fluid stream. The device is small, simple, and disposable yet is an integrated system complete with sample inlets, outlets, sorting channel, and a novel passively driven pumping system that provides a steady flow of liquid; it requires no external power source or controls. The device fulfills a need in clinical settings where small amounts of sperm need to be sorted. It also opens the way for convenient bioassays based on sperm motility including at-home motile sperm tests.
374 citations
TL;DR: In this article, a MoFlo flow cytometer/cell sorter was used to test the DNA-binding dye Hoechst 33342 on fresh sperm collected from 22 bulls of unknown fertility of various beef and dairy breeds.
324 citations
TL;DR: It has yet to be determined whether seminal plasma would have similar effects on flow cytometrically sorted spermatozoa of other species, and what its effects might be on the in vivo fertility of flow sorted boar.
306 citations
TL;DR: Sexed sperm on demand over the next several years will provide livestock producers with many options in seeking to improve efficiency of production and improve quality of products to enhance consumer acceptability.
302 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 14 |
| 2020 | 9 |
| 2019 | 15 |
| 2018 | 6 |
| 2017 | 4 |
| 2016 | 9 |