Topic
Smithia
About: Smithia is a research topic. Over the lifetime, 7 publications have been published within this topic receiving 12 citations.
Papers
TL;DR: The ITS barcode indicates that S. conferta and Smithia sensitiva are closely related compared to other species, and further chloroplast barcodes should be tested for phylogenetic analysis of genus Smithia.
Abstract: Background: Smithia conferta Sm. is an annual herb widely used in Indian traditional medical practice and commonly known as “Lakshman booti” in Sanskrit. Morphological resemblance among the species of genus Smithia Aiton. leads to inaccurate identification and adulteration. This causes inconsistent therapeutic effects and also affects the quality of herbal medicine. Aim: This study aimed to generate potential barcode for authentication of S. conferta and its adulterants through DNA barcoding technique. Materials and Methods: Genomic DNA extracted from S. conferta and its adulterants was used as templates for polymerase chain reaction amplification of the barcoding regions. The amplicons were directed for sequencing, and species identification was conducted using BLASTn and unweighted pair-group method with arithmetic mean trees. In addition, the secondary structures of internal transcribed spacer (ITS) 2 region were predicted. Results: The nucleotide sequence of ITS provides species-specific single nucleotide polymorphisms and sequence divergence (22%) than psbA-trnH (10.9%) and rbcL (3.1%) sequences. The ITS barcode indicates that S. conferta and Smithia sensitiva are closely related compared to other species. Conclusion: ITS is the most applicable barcode for molecular authentication of S. conferta, and further chloroplast barcodes should be tested for phylogenetic analysis of genus Smithia. Abbreviations used: BLASTn: Basic Local Alignment Search Tool for Nucleotide; MEGA: Molecular Evolutionary Genetic Analysis; EMBL: European Molecular Biology Laboratory; psbA-trnH: Photosystem II protein D1- stuctural RNA: His tRNA gene; rbcL: Ribulose 1,5 bi-phosphate carboxylase/ oxygenase large subunit gene.
13 citations
2 Sep 2020
TL;DR: The most complete DNA sequence data is assembled and Bayesian and maximum likelihood phylogenetic analyses unequivocally confirm the non-monophyly of Aeschynomene.
Abstract: The pantropical and morphologically diverse genus Aeschynomene (Leguminosae, Papilionoideae) comprises ca. 180 species, but its monophyly has never been comprehensively addressed in a molecular phylogenetic context of the dalbergioid clade. Here we have assembled the most complete DNA sequence data (nuclear ribosomal ITS/5.8S and the plastid genes trnL intron and protein-coding matK), representatively sampled both in Aeschynomene and the closely related Dalbergia and Machaerium, as well all currently accepted genera from the dalbergioids. The Bayesian and maximum likelihood phylogenetic analyses unequivocally confirm the non-monophyly of Aeschynomene. The pantropically distributed Aeschynomene sect. Aeschynomene, often inhabiting riparian and wetland habitats, is paraphyletic with respect to the mostly African genera Bryaspis, Cyclocarpa, Geissaspis, Humularia, Kotschya, and Smithia, as well as the South American monotypic genus Soemmeringia. The strongly supported and morphologically coherent clade comprising the American species traditionally classified within Aeschynomene sect. Ochopodium, with a greater ecological predilection for neotropical fire-prone savannas and the seasonally dry tropical forests and woodlands, is here elevated to generic rank. As such, this lineage is re-established as the previously monotypic genus Ctenodon. A complete description of Ctenodon and 78 new combinations for the American species and associated infraspecific taxa are provided, as well as 24 lectotypifications.
12 citations
1 Nov 2011
TL;DR: In this paper, the cucumber fruit juice inhibit bacterial growth A. smithia salmonicida by using eksperimental method by in vitro, with a large measure of cucumber juice inhibition zone around the existing paper disc and comparing it to a large zone of inhibition using ampicillin.
Abstract: Giving antibiotics continuously can cause A. salmonicida smithia become resistant and residues of antibiotics may pollute the water environment. One alternative to using traditional medicinal plants that are antibacterial. Advantages of using traditional medicinal plants are relatively more secure, easily available, inexpensive, does not cause resistance, and relatively harmless to the surrounding environment. The content of natural chemicals from cucumber fruit is known to have antibacterial effects namely, flavonoids and phenols. The purpose of this study to determine ability the cucumber fruit juice inhibit bacterial growth A. salmonicida smithia and to determine the best concentration to inhibit bacterial growth A. salmonicida smithia with ampicillin drugs for comparison by in vitro. This research was conducted in the Bacteriology Laboratory of Fish Quarantine Center of Sidoarjo and Human Genetic Laboratory the Institute of Tropical Disease Centre, Airlangga University, Surabaya. This research use done eksperimental method by in vitro, with a large measure of cucumber fruit juice inhibition zone around the existing paper disc and comparing it to a large zone of inhibition using ampicillin. Concentration dilution series cucumber fruit juice used during the research, began 2 gr/ 2 ml to the concentration of 0,0625 gr/ml. Result of research show at concentration 0,125 gr/ml still has ability to pursue bacterium A. smithia salmonicida, so that can be anticipated that effective cucumber fruit juice if used as by drug, because at small concentration can to pursue bacteria A. smithia salmonicida by in vitro.
4 citations
3 citations
1 Jan 1990
TL;DR: The tribe Aeschynomeneae has been revised and keys to the subtribes, genera and species (in each genus) and distributional maps of the species are provided.
Abstract: The tribe Aeschynomeneae has been revised. Seven genera, viz Aeschynomene L. (6 species), Arachis L. (1 species), Cyclocarpa Afzelius ex Urban (1 species), Ormocarpum P. Beauv. (1 species), Smithia Aiton (2 species), Stylosanthes Swartz (5 species) and Zornia J. Gmelin (17 species), occur in Australia. With the exception of Zornia (discussed previously) all genera are dealt with in this paper. All taxa are described and keys to the subtribes, genera and species (in each genus) and distributional maps of the species are provided. No new taxa are described in this account.
1 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2020 | 1 |
| 2017 | 1 |
| 2016 | 1 |
| 2014 | 1 |
| 2011 | 1 |
| 1990 | 1 |