TL;DR: Levels of genetic divergence suggested by the cpDNA data were consistent with cytodeme recognition in the subtribe, but provided evidence for inconsistencies in the current generic delimitations based on morphology.
Abstract: Chloroplast DNA restriction sites for 20 endonucleases were mapped using cpDNA probes from Brassica juncea and site variation was surveyed in 33 diploid taxa of the Subtribe Brassicinae. A total of 419 mutations was observed, including both site (i.e., gain/ loss) and fragment length (i.e., insertions or deletions); 221 (53%) mutations showed variation at the interspecific level. Phylogenetic analysis indicated a clear division of the subtribe into two ancient evolutionary lineages. These were (I) the “Nigra” lineage: Brassica nigra, B. fruticulosa, B. tournefortii, Sinapis pubescens, S. alba, S. flexuosa, S. arvensis, Coincya cheiranthos, Erucastrum canariense, and Hirschfeldia incana, and (II) the “Rapa/ Oleracea” lineage: Brassica rapa, B. oleracea ssp. oleracea and ssp. alboglabra, B. rupestris-villosa complex (B. rupestris, B. drepanensis, B. macrocarpa, B. villosa), B. barrelieri, B. deflexa, B. oxyrrhina, B. gravinae, Diplotaxis erucoides, D. tenuifolia, Eruca sativa, Raphanus raphanistrum, R. sativus, and Sinapis aucheri. In the “Nigra” lineage, Brassica nigra was most closely related to the annual Sinapis species, S. arvensis and S. alba. In the “Rapa/Oleracea” lineage, the Brassica rapa and B. oleracea genomes formed a distinct group whose closest relatives were the wild species of the B. oleracea (n=9) complex (i.e., B. rupestris-villosa complex). Species with n=7 chromosomes exist in both lineages. Hirschfeldia incana (n=7), in the “Nigra” lineage, was most closely related to Sinapis pubescens. In the “Rapa/Oleracea” lineage three taxa with n=7 — B. deflexa, D. erucoides, and S. aucheri — were closely related, advanced in the lineage, and were the closest apparent relatives (particularly D. erucoides) to B. rapa, B. oleracea, and its wild relatives. Levels of genetic divergence suggested by the cpDNA data were consistent with cytodeme recognition in the subtribe, but provided evidence for inconsistencies in the current generic delimitations based on morphology. Very low levels of genetic divergence were evident among taxa/accessions within a cytodeme. Raphanus was closely related to the Brassica rapa and B. oleracea genomes and clearly belongs in Subtribe Brassicinae. Several cytoplasmic genetic markers of potential use in plant breeding programs were identified for each of the cytodemes.
TL;DR: The results suggest that the contribution of photosynthesis to light-dependent high irradiance reaction anthocyanin synthesis in seedlings of cabbage, mustard, tomato, and turnip is minimal, if any at all.
Abstract: Streptomycin enhances the synthesis of anthocyanins and inhibits the synthesis of chlorophylls and the development of chloroplasts in dark-grown seedlings of cabbage (Brassica oleracea), mustard (Sinapis alba), tomato (Lycopersicon esculentum), and turnip (Brassica rapa) exposed to prolonged periods of irradiation in various spectral regions. These results suggest that the contribution of photosynthesis to light-dependent high irradiance reaction anthocyanin synthesis in seedlings of cabbage, mustard, tomato, and turnip is minimal, if any at all. So far, phytochrome is the only photoreceptor whose action in the control of light-dependent anthocyanin synthesis in seedlings of cabbage, mustard, tomato, and turnip has been satisfactorily demonstrated.
TL;DR: The genus Brassica is one of 51 genera in the tribe Brassiceae belonging to the crucifer family, and is the economically most important genus within this tribe, containing 37 different species (Gomez-Campo 1980).
Abstract: The genus Brassica is one of 51 genera in the tribe Brassiceae belonging to the crucifer family, and is the economically most important genus within this tribe, containing 37 different species (Gomez-Campo 1980). Many crop species are included in the Brassica genus, which provide edible roots, leaves, stems, buds, flowers and seed. Next in agronomic importance are the genera Raphanus, cultivated for its edible roots and Sinapis as a source of condiments. There are many wild relatives that have potential as sources for oil, condiments and other products. Wild relatives could serve as sources for cytoplasmic male sterility (androsterility) for the development of hybrid seed production systems in Brassica crop plants and provide nuclear genes for resistance to different diseases and pests. Certain visible characters are informative in the Brassiceae. Mucilage is a very common phenomenon in seed of plants of the tribe Brassiceae, and is therefore of taxonomic value. For instance, seeds of the 9 chromosome species of the genus Sinapis such as S. arvensis contain no mucilage, while the 12 chromosome species such as S. alba contain mucilage. Some species of Sinapis and Crambe have hairy cotyledons, which is an exception. Drought-adapted genera such as Moricandia tend to be glabrous, but they are more sensitive to aphids than hairy species. There is a wide variation in flower shape and colour from yellow to white to violet (Moricandia), and the colour of mature seeds varies from yellow to black.
TL;DR: What is known about the predominant storage proteins of commercially produced Brassicaceae seeds relative to the chemistry, nutritional value, as well as the functionality in foods, and associated non-protein components of canola/rapeseed storage proteins is critically reviewed.
Abstract: Among the commercially cultivated Brassicaceae (Cruciferae) plants, Brassica juncea, Brassica napus, Brassica rapa, and Sinapis alba store significant amounts of oil and protein in the seed. At present, Brassica seed proteins are primarily used for livestock feeding based on the nutritional value. The point of curiosity is whether the present knowledge on the protein structure, biochemical characteristics, nutritive value, and the recovery processes are inadequate to develop Brassica proteins into a usable plant protein source or these proteins are of substandard for uses beyond animal nutrition applications. Cruciferin (11S) and napin (2S) are the predominant storage proteins of Brassicaceae seeds that contribute to different properties and functions. A gamut of information is available on the chemistry, nutritional value, as well as the functionality in foods, and associated non-protein components of canola/rapeseed storage proteins. The intention of this article is to critically review what is known about the predominant storage proteins of commercially produced Brassicaceae seeds relative to the above aspects and identify the knowledge gaps.
TL;DR: An in vitro study has conducted of the nematocidal effect of some glucosinolates and the products of their myrosinase-mediated enzymatic hydrolysis on second-stage juveniles of the sugar beet cyst nematode Heterodera schachtii.
Abstract: An in vitro study has conducted of the nematocidal effect of some glucosinolates and the products of their myrosinase-mediated enzymatic hydrolysis on second-stage juveniles of the sugar beet cyst nematode Heterodera schachtii. The glucosinolates tested were purified from the seeds or from plant organs of some crucifers cited as hosts of the nematode: Brassica napus, Lepidium sativum, Brassica rapa, Brassica carinata, Raphanus sativus, and Sinapis alba. The glucosinolates were dissolved concentrations in both the absence and presence of the enzyme myrosinase, and their nematocidal effect in time on second-stage juveniles was then determined