Topic
Shotgun
About: Shotgun is a research topic. Over the lifetime, 174 publications have been published within this topic receiving 1190 citations. The topic is also known as: scattergun & peppergun.
Papers published on a yearly basis
1 / 2
Papers
1 Jan 2020
TL;DR: Shotgun metagenomic sequencing of the Arctic Ocean during winter-spring transition to assess the impact of thinner sea ice on the microbiome structure and function.
Abstract: One of the main concerns about the Arctic Ocean has been the changing sea ice regime with a reduction in the summer sea ice extent and a shift in dominance from thicker, perennial multiyear ice towards thinner, first-year ice. As the dietary basis of marine food webs and central players of biogeochemical cycles, microbial communities play an irreplaceable role when evaluating the ecological impact of the Arctics thinner ice regime. During the Norwegian young sea Ice cruise 2015 (N-ICE2015), that took place in drifting pack ice north of Svalbard between January-June 2015, seawater was collected, at 5, 20 or 50, 250 m depth in 9th March, 27th April and 16th June, together with physical and biogeochemical data. Through the massively parallel sequencing of environmental DNA (metagenomics) we expect to get a snapshot of the Arctics microbiome structure, key functions and dynamic through the dark-light transition.
92 citations
84 citations
83 citations
TL;DR: The design, implementation and operation of the "compartmentalized shotgun assembler" is described, which makes use of preliminary data produced by both approaches to determine the human genome.
Abstract: Two different strategies for determining the human genome are currently being pursued: one is the “clone-byclone” approach, employed by the publicly funded project, and the other is the “whole genome shotgun assembler” approach, favored by researchers at Celera Genomics. An interim strategy employed at Celera, called compartmentalized shotgun assembly, makes use of preliminary data produced by both approaches. In this paper we describe the design, implementation and operation of the “compartmentalized shotgun assembler”.
67 citations
TL;DR: This work briefly reviews the recent development of chromatographic techniques and methods in shotgun proteome analysis, including the following three aspects: one-dimensional separation; multidimensional separation; and, automated proteome-analysis systems.
Abstract: Shotgun proteomics is a high-throughput approach to proteome analysis whereby the protein mixture is digested and the peptides generated are separated by capillary liquid chromatography and sequenced by tandem mass spectrometry (MS). Due to the huge number of peptide species, separation prior to MS analysis plays an important role in shotgun proteomics. Overall sensitivity, dynamic range, throughput and general effectiveness of shotgun proteomic analysis largely depend on how well the peptide mixture is separated. In recent years, new separation techniques have been applied successfully to proteome analysis and have dramatically improved protein identification. We briefly review the recent development of chromatographic techniques and methods in shotgun proteome analysis, including the following three aspects: one-dimensional separation; multidimensional separation; and, automated proteome-analysis systems.
63 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 96 |
| 2024 | 139 |
| 2023 | 123 |
| 2022 | 140 |
| 2021 | 80 |
| 2020 | 52 |