Topic
Shaker
About: Shaker is a research topic. Over the lifetime, 2178 publications have been published within this topic receiving 31219 citations.
Papers published on a yearly basis
1 / 3
Papers
TL;DR: In this paper, the authors reported the crystal structure of a mammalian voltage-dependent potassium ion (K+) channel, Kv1.2, which is a member of the Shaker K+ channel family.
Abstract: Voltage-dependent potassium ion (K+) channels (Kv channels) conduct K+ ions across the cell membrane in response to changes in the membrane voltage, thereby regulating neuronal excitability by modulating the shape and frequency of action potentials. Here we report the crystal structure, at a resolution of 2.9 angstroms, of a mammalian Kv channel, Kv1.2, which is a member of the Shaker K+ channel family. This structure is in complex with an oxido-reductase beta subunit of the kind that can regulate mammalian Kv channels in their native cell environment. The activation gate of the pore is open. Large side portals communicate between the pore and the cytoplasm. Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit.
2,249 citations
TL;DR: A region near the amino terminus with an important role in inactivation has been identified and the results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.
Abstract: The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes. A region near the amino terminus with an important role in inactivation has now been identified. The results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.
1,602 citations
TL;DR: Two of the complementary DNA clones have been sequenced and their sequences support the hypothesis that Shaker encodes a component of a K+ channel, the A channel of Drosophila melanogaster.
Abstract: On the basis of electrophysiological analysis of Shaker mutants, the Shaker locus of Drosophila melanogaster has been proposed to encode a structural component of a voltage-dependent potassium channel, the A channel. Unlike sodium channels, acetylcholine receptors, and calcium channels, K+ channels have not been purified biochemically. To facilitate biochemical studies of a K+ channel, genomic DNA from the Shaker locus has been cloned. Rearrangements in five Shaker mutants have been mapped to a 60-kilobase segment of the genome. Four complementary DNA clones have been analyzed. These clones indicate that the Shaker gene contains multiple exons distributed over at least 65 kilobases of genomic DNA in the region where the mutations mapped. Furthermore, the gene may produce several classes of alternatively spliced transcripts. Two of the complementary DNA clones have been sequenced and their sequences support the hypothesis that Shaker encodes a component of a K+ channel.
769 citations
TL;DR: Findings show that movement of the NH2- terminal half but not the CO2H-terminal end of the S4 segment underlies gating charge, and that this portion of theS4 segment appears to move across the entire transmembrane voltage difference in association with channel activation.
719 citations
TL;DR: The results indicate that conserved charged amino acids in putative transmembrane segments S2, S3, and S4 contribute to the gating charge of the channel and are a major component of the voltage sensor.
704 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 37 |
| 2024 | 82 |
| 2023 | 150 |
| 2022 | 200 |
| 2021 | 52 |
| 2020 | 58 |