Topic
Sequence Inversion
About: Sequence Inversion is a research topic. Over the lifetime, 19 publications have been published within this topic receiving 677 citations.
Papers
TL;DR: Closed circular Moloney murine leukemia virus (M-MuLV) DNA was prepared from recently infected cells and cloned in a lambda vector, providing explicit information concerning the mechanism by which retrovirus DNA integrates into host cell DNA.
Abstract: Closed circular Moloney murine leukemia virus (M-MuLV) DNA was prepared from recently infected cells and cloned in a lambda vector. Four classes of cloned M-MuLV inserts were found: Class I, full length 8.8-kilobase (kb) inserts with two tandem long terminal repeats (LTRs) of 600 base pairs; class 2, 8.2-kb inserts with a single copy of a LTR; class 3, M-MuLV DNA inserts with various portions deleted; and class 4, an 8.8-kb insert with an internal sequence inversion. Determination of nucleotide sequence at the junction between the two LTRs from a class 1 insert suggested that circularization occurred by blunt-end ligation of an 8.8-kb linear DNA. The class 4 molecule had an inversion that was flanked by inverted LTRs, each of which had lost two terminal base pairs at the inversion end points. Also, four base pairs that were present only once in standard M-MuLV DNA were duplicated at either end of the inversion. This molecule was interpreted as resulting from an integrative inversion in which M-MuLV DNA has integrated into itself. Its analysis thus provided explicit information concerning the mechanism by which retrovirus DNA integrates into host cell DNA. Models of retrovirus integration based on bacterial DNA transposition mechanisms are proposed.
288 citations
TL;DR: Inversion of the L and S components of the HSV-1 genome during productive infection does not appear to be a site-specific process, but rather is the result of generalized recombination mediated by the complex of gene products that replicate the viral DNA.
100 citations
TL;DR: Double-stranded cDNA has been synthesized from influenza virus RNA and cloned into derivatives of the bacteriophage M13 for sequence analysis and inversions of sequence within the same cDNA molecule were the predominant type of rearrangement.
74 citations
Journal Article•
TL;DR: A stand alone, platform independent, graphic alignment tool for comparative sequence analysis that uses the NCBI-BLASTN program and extensive post-processing to exhaustively align two DNA sequences and provides researchers with a fine-grained alignment and visualization tool aptly suited for non-coding, 0–200 kb, pairwise, sequence analysis.
Abstract: Several problems exist with current methods used to align DNA sequences for comparative sequence analysis. Most dynamic programming algorithms assume that conserved sequence elements are collinear. This assumption appears valid when comparing orthologous protein coding sequences. Functional constraints on proteins provide strong selective pressure against sequence inversions, and minimize sequence duplications and feature shuffling. For non-coding sequences this collinearity assumption is often invalid. For example, enhancers contain clusters of transcription factor binding sites that change in number, orientation, and spacing during evolution yet the enhancer retains its activity. Dot plot analysis is often used to estimate non-coding sequence relatedness. Yet dot plots do not actually align sequences and thus cannot account well for base insertions or deletions. Moreover, they lack an adequate statistical framework for comparing sequence relatedness and are limited to pairwise comparisons. Lastly, dot plots and dynamic programming text outputs fail to provide an intuitive means for visualizing DNA alignments.
45 citations
Patent•
13 Sep 1999TL;DR: In this paper, an active matrix device with inversion circuitry coupled with a Cole sequence generator has been proposed to reduce the direct current bias voltage applied by the electronic element to the sub-pixel.
Abstract: An active matrix device has a plurality of drive signals, a plurality of select signals and an array of sub-pixels. Each of the sub-pixels has an electronic element connected to one of the drive signals and one of the select signals to display. The active matrix device also includes inversion circuitry coupled to the drive signals that has at least one Cole sequence generator. A Cole sequence generator provides a random, semi-random or pseudo-random sequence pattern. The inversion circuitry is capable of reducing the direct current bias voltage applied by the electronic element to the sub-pixel. The inversion circuitry is further capable of reducing flicker of the active matrix device.
37 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2017 | 2 |
| 2016 | 2 |
| 2015 | 1 |
| 2014 | 1 |
| 2005 | 2 |
| 1999 | 1 |