Topic
Septin ring
About: Septin ring is a research topic. Over the lifetime, 155 publications have been published within this topic receiving 246579 citations.
Papers published on a yearly basis
Papers
TL;DR: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
Abstract: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae. Transplacement vectors were constructed and used to derive yeast strains containing nonreverting his3, trp1, leu2 and ura3 mutations. A set of YCp and YIp vectors (pRS series) was then made based on the backbone of the multipurpose plasmid pBLUESCRIPT. These pRS vectors are all uniform in structure and differ only in the yeast selectable marker gene used (HIS3, TRP1, LEU2 and URA3). They possess all of the attributes of pBLUESCRIPT and several yeast-specific features as well. Using a pRS vector, one can perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
9,205 citations
TL;DR: This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast.
Abstract: This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.
2,674 citations
TL;DR: Two features which distinguish the cell cycle of Saccharomyces cerevisiae from most other eukaryotes are particularly useful for an analysis of the gene functions that control the cell division cycle.
Abstract: Mitotic cell division in eukaryotes is accomplished through a highly reproducible temporal sequence of events that is common to almost all higher organisms. An interval of time, Gl, separates the previous cell division from the initiation of DNA synthesis. Ohromosome replication is acco’mplished during the DNA synthetic period, S, which typically occupies about a third of the cell cycle. Another interval of time, G2, separates the completion of DNA synthesis from prophase, the beginning of mitosis, M. A dramatic sequence of changes in chromosome structure and of chromosome ,movement characterizes the brief mitotic period that results in the precise separation of sister chromatids to daughter nuclei. Mitosis is followed by cytokinesis, the partitioning of the cytoplasm into two daughter cells with separate plasma membranes. In some organisms the cycle is completed by cell wall separation. Each of these events occurs during the cell division cycle of the yeast, Saccharomyces cerevisiae (I) (Fig. 1) . However, two features which distinguish the cell cycle of S. cerevisiae from most other eukaryotes are particularly useful for an analysis of the gene functions that control the cell division cycle. First, the fact that both haploid and diploid cells undergo mitosis permits the isolation of recessive mutations in haploids and their analysis by complementation in diploids. Second, the daughter cell is recognizable at an early stage of the cell cycle as a bud on the surface of the parent cell. Since the ratio of bud size to parent cell size increases progressively during the cycle, this ratio pro-
1,826 citations
TL;DR: It is concluded that bud emergence is not a necessary prerequisite for the completion of DNA replication but is apparently necessary for nuclear division and Cytokinesis and cell separation are not necessary prerequisites for bud emergence, DNA replication, or nuclear division.
700 citations
TL;DR: It is concluded that septin alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that sePTins help organize these bundles.
555 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 5 |
| 2020 | 7 |
| 2019 | 4 |
| 2018 | 4 |
| 2017 | 4 |
| 2016 | 3 |