Topic
Sepal morphogenesis
About: Sepal morphogenesis is a research topic. Over the lifetime, 2 publications have been published within this topic receiving 68 citations.
Papers
TL;DR: This investigation further examined regulation of putative developmental genes possibly involved in tomato fruit ripening and development and revealed differences in gene expression that may be important for tomato cultivar variation.
Abstract: Red ripe tomatoes are the result of numerous physiological changes controlled by hormonal and developmental signals, causing maturation or differentiation of various fruit tissues simultaneously. These physiological changes affect visual, textural, flavor, and aroma characteristics, making the fruit more appealing to potential consumers for seed dispersal. Developmental regulation of tomato fruit ripening has, until recently, been lacking in rigorous investigation. We previously indicated the presence of up-regulated transcription factors in ripening tomato fruit by data mining in TIGR Tomato Gene Index. In our in-vitro system, green tomato sepals cultured at 16 to 22°C turn red and swell like ripening tomato fruit while those at 28°C remain green. Here, we have further examined regulation of putative developmental genes possibly involved in tomato fruit ripening and development. Using molecular biological methods, we have determined the relative abundance of various transcripts of genes during in vitro sepal ripening and in tomato fruit pericarp at three stages of development. A number of transcripts show similar expression in fruits to RIN and PSY1, ripening-associated genes, and others show quite different expression. Our investigation has resulted in confirmation of some of our previous database mining results and has revealed differences in gene expression that may be important for tomato cultivar variation. We present new and intriguing information on genes that should now be studied in a more focused fashion.
52 citations
TL;DR: In this paper, 1-methylcyclopropene was used to block ethylene-induced gene expression by interrupting the ethylene signal transduction pathway that occurs in ripening tomato fruits and presumably in vine sepals.
Abstract: Tomato (Solanum lycopersicum, formerly Lycopersicon esculentum) cv. VFNT Cherry sepals, when cultured in vitro between 16 degrees C and 22 degrees C, change their genetic programme to that of ripening fruit. Previously regulation of a number of transcription factors and a putative G-protein-coupled receptor that may be involved in tomato fruit ripening and cool-temperature sepal morphogenesis had been revealed. Many of those genes such as TAG1, TM4, TM6, AP2-like (LeAP2FR), YABBY2-like (LeYAB2), and LeCOR413-PM1 have not been investigated for ethylene regulation. Ethylene-independent, regulated transcripts may be part of an early signalling process induced or de-repressed by cool temperature that causes a switch in the genetic programme of the sepals. In this paper, ethylene regulation of a number of these and other putative signalling factors are investigated during cool-temperature-induced sepal morphogenesis. 1-Methylcyclopropene was used to block ethylene-induced gene expression by interrupting the ethylene signal transduction pathway that occurs in ripening tomato fruits and presumably in ripening sepals. Transcripts of several putative transcription factors previously shown to be up-regulated during cool-temperature-induced sepal morphogenesis (TAG1, TM4, LeAP2FR) were only slightly or not induced in 1-methylcyclopropene-treated sepals, indicating either direct or indirect ethylene regulation. Two genes, VAHOX1, a homeobox domain leucine-zipper-encoding gene, and LeYAB2, a putative zinc-finger transcription factor-encoding gene, increased in treated and untreated sepals indicating regulation by cool temperatures independently of ethylene.
37 citations
Performance Metrics
| Year | Papers |
|---|---|
| 2007 | 1 |
| 2003 | 1 |