Topic
SEMA3A
About: SEMA3A is a research topic. Over the lifetime, 374 publications have been published within this topic receiving 32779 citations.
Papers published on a yearly basis
Papers
TL;DR: It is reported that neuropilin, a type I transmembrane protein implicated in aspects of neurodevelopment, is a Sema III receptor and the identification of neuropILin-2, a related neuro pilin family member, is described.
1,261 citations
TL;DR: It is concluded that plexins are receptors for multiple (and perhaps all) classes of semaphorins, either alone or in combination with neuropilins, and trigger a novel signal transduction pathway controlling cell repulsion.
1,225 citations
TL;DR: Evidence is provided that neuropilin is a receptor or a component of a receptor complex that mediates the effects of Sema III on these axons, and that antibodies to neuro pilin block the ability ofSema III to repel sensory axons and to induce collapse of their growth cones.
1,210 citations
TL;DR: The cloning and characterization of two sema genes in Drosophila, one in human, and the identification of two related viral sequences are reported, all of which encode proteins with conserved Semaphorin domains.
1,007 citations
TL;DR: In this paper, the authors investigated the neuro-regenerative potential of Sema3A on adult peripheral nervous system neurons such as those that innervate the cornea and found that upon cornea injury, there is a fast increase in Semaphorin3A expression.
Abstract: The peripheral sensory nerves that innervate the cornea can be easily damaged by trauma, surgery, infection or diabetes. Several growth factors and axon guidance molecules, such as Semaphorin3A (Sema3A) are upregulated upon cornea injury. Nerves can regenerate after injury but do not recover their original density and patterning. Sema3A is a well known axon guidance and growth cone repellent protein during development, however its role in adult cornea nerve regeneration remains undetermined. Here we investigated the neuro-regenerative potential of Sema3A on adult peripheral nervous system neurons such as those that innervate the cornea. First, we examined the gene expression profile of the Semaphorin class 3 family members and found that all are expressed in the cornea. However, upon cornea injury there is a fast increase in Sema3A expression. We then corroborated that Sema3A totally abolished the growth promoting effect of nerve growth factor (NGF) on embryonic neurons and observed signs of growth cone collapse and axonal retraction after 30 min of Sema3A addition. However, in adult isolated trigeminal ganglia or dorsal root ganglia neurons, Sema3A did not inhibited the NGF-induced neuronal growth. Furthermore, adult neurons treated with Sema3A alone produced similar neuronal growth to cells treated with NGF and the length of the neurites and branching was comparable between both treatments. These effects were replicated in vivo, where thy1-YFP neurofluorescent mice subjected to cornea epithelium debridement and receiving intrastromal pellet implantation containing Sema3A showed increased corneal nerve regeneration than those receiving pellets with vehicle. In adult PNS neurons, Sema3A is a potent inducer of neuronal growth in vitro and cornea nerve regeneration in vivo. Our data indicates a functional switch for the role of Sema3A in PNS neurons where the well-described repulsive role during development changes to a growth promoting effect during adulthood. The high expression of Sema3A in the normal and injured adult corneas could be related to its role as a growth factor.
889 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 7 |
| 2024 | 4 |
| 2023 | 30 |
| 2022 | 32 |
| 2021 | 8 |
| 2020 | 17 |