Topic
SECIS element
About: SECIS element is a research topic. Over the lifetime, 167 publications have been published within this topic receiving 12873 citations.
Papers published on a yearly basis
Papers
TL;DR: This work identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs.
Abstract: In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are misannotated. We identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs. The human selenoproteome consists of 25 selenoproteins.
2,365 citations
TL;DR: The regulation of translation frequently involves protein-RNA interactions, and in prokaryotes a single RNA-binding protein, a selenocysteine-specific elongation factor, interacts with both the tRNA and mRNA to confer decoding.
456 citations
TL;DR: The presence of SECIS elements in eukaryotic selenoprotein mRNAs permits complete flexibility in UGA codon position.
Abstract: We investigated the requirements for selenocysteine insertion at single or multiple UGA codons in eukaryotic selenoproteins Two functional SECIS elements were identified in the 3' untranslated region of the rat selenoprotein P mRNA, with predicted stem-loops and critical nucleotides similar to those in the SECIS elements in the type I iodothyronine 5' deiodinase (5'DI) and glutathione peroxidase selenoprotein mRNAs Site-directed mutational analyses of three SECIS elements confirmed that conserved nucleotides in the loop and in unpaired regions of the stem are critical for activity This indicates that multiple contact sites are required for SECIS function Stop codon function at any of five out-of-context UGA codons in the 5'DI mRNA was suppressed by SECIS elements from the 5'DI or selenoprotein P genes linked downstream Thus, the presence of SECIS elements in eukaryotic selenoprotein mRNAs permits complete flexibility in UGA codon position
446 citations
TL;DR: It is established that SBP2 is essential for the co‐translational insertion of Sec into selenoproteins and hypothesize that the binding activity of SBp2 may be involved in preventing termination at the UGA/Sec codon.
Abstract: In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) element in the 3' untranslated region of the mRNA. We purified a SECIS binding protein, SBP2, and obtained a cDNA clone that encodes this activity. SBP2 is a novel protein containing a putative RNA binding domain found in ribosomal proteins and a yeast suppressor of translation termination. By UV cross-linking and immunoprecipitation, we show that SBP2 specifically binds selenoprotein mRNAs both in vitro and in vivo. Using (75)Se-labeled Sec-tRNA(Sec), we developed an in vitro system for analyzing Sec incorporation in which the translation of a selenoprotein mRNA was both SBP2 and SECIS element dependent. Immunodepletion of SBP2 from the lysates abolished Sec insertion, which was restored when recombinant SBP2 was added to the reaction. These results establish that SBP2 is essential for the co-translational insertion of Sec into selenoproteins. We hypothesize that the binding activity of SBP2 may be involved in preventing termination at the UGA/Sec codon.
410 citations
TL;DR: In this review, an overview of eukaryotic selenoproteins and selenobroteomes is provided, which are sets of seleno-based proteins in these organisms, which show a mosaic occurrence, with some organisms, such as vertebrates and algae, having dozens of these proteins, while other organisms having lost all seleniproteins during evolution.
347 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 2020 | 2 |
| 2019 | 2 |
| 2018 | 4 |
| 2017 | 3 |
| 2016 | 2 |