TL;DR: Mating calls are known for 29 species of African Bufo belonging to 11 species groups, and geographical distribution of three call types indicates large radiations of one or two call types in South America, North America and Africa.
Abstract: Mating calls are known for 29 species of African Bufo belonging to 11 species groups. Twenty-five African species, representing eight species groups (including four groups or complexes having 2N=22) have calls which Martin (1972) termed Type I. This call type is also found in Schismaderma carens, Nectophrynoides tornieri and N. occidentalis. It is known in only four species of Bufo outside Africa and in Odontophrynus americanus which is thought to be closely related to leptodactylids that gave rise to the genus Bufo. Four African species of Bufo have Type II calls.Geographic distribution of three call types indicates large radiations of one or two call types in South America, North America and Africa. The European and Asian Bufo faunas appear to be derived primarily from American radiations.The radiation of bufonids in Africa appears to be equal to that of South America. An explanation of this may be that Bufo or its progenitor evolved prior to the continental separation of South America and Africa.
TL;DR: In a sample of 27 toads, 37% were infected with up to 130 parasites per host (mean intensity 37).
Abstract: Eupolystoma vanasi is described as a new species of the Polystomatidae parasitic in the urinary bladder of Schismaderma carens in Northern Province and KwaZulu-Natal Province, South Africa. This is the third Eupolystoma species described from Africa and the first polystomatid from Schismaderma, an anuran genus that is primitive with respect to the other African bufonids in which Eupolystoma has been recorded. The species is distinguished by body size (this is the largest Eupolystoma known; mean length of adults 6 mm), by genital spine number (4 in comparison with 6-9 in other species), marginal hooklet length (greater than in other African species), and by the small size of the ovary and testis. In a sample of 27 toads, 37% were infected with up to 130 parasites per host (mean intensity 37). Worm burdens of this magnitude are exceptional amongst polystomatids in general but are characteristic of Eupolystoma, where there may be repeated re-infection of adult hosts and, uniquely, a direct, internal cycle of auto-infection.