TL;DR: The results suggest that the genetic diversity of trout in the Ponto‐Caspian region is best explained with the fragmentation of catchments, and a conservative taxonomic approach is suggested.
Abstract: Current taxonomy of western Eurasian trout leaves a number of questions open; it is not clear to what extent some species are distinct genetically and morphologically. The purpose of this paper was to explore phylogeography and species boundaries in freshwater and anadromous trout from the drainages of the Black and the Caspian Seas (Ponto-Caspian). We studied morphology and mitochondrial phylogeny, combining samples from the western Caucasus within the potential range of five nominal species of trout that are thought to inhabit this region, and using the sequences available from GenBank. Our results suggest that the genetic diversity of trout in the Ponto-Caspian region is best explained with the fragmentation of catchments. (1) All trout species from Ponto-Caspian belong to the same mitochondrial clade, separated from the other trout since the Pleistocene; (2) the southeastern Black Sea area is the most likely place of diversification of this clade, which is closely related to the clades from Anatolia; (3) The species from the Black Sea and the Caspian Sea drainages are monophyletic; (4) except for the basal lineage of the Ponto-Caspian clade, Salmo rizeensis, all the lineages produce anadromous forms; (5) genetic diversification within the Ponto-Caspian clade is related to Pleistocene glacial waves; (6) the described morphological differences between the species are not fully diagnostic, and some earlier described differences depend on body size; the differences between freshwater and marine forms exceed those between the different lineages. We suggest a conservative taxonomic approach, using the names S. rizeensis and Salmo labrax for trout from the Black Sea basin and Salmo caspius and Salmo ciscaucasicus for the fish from the Caspian basin.
TL;DR: Bu calismada, Karadeniz alabaligi’in besinci neslinin kultur karakteristikleri belirlenmistir kapali devre yetistiriclik unitesi (RAS)’nde uretilmis ve smolt boya gelene dek burada tutulmuslardir.
Abstract: In this research, the culture characteristics of the fifth generation of Black Sea trout ( Salmo trutta labrax PALLAS, 1814) were evaluated. First of all, trout were cultured in recirculating aquaculture system (RAS) and they kept in RAS until smoltification. After that, they were transported to marine cages till market size. Thus, the ratios of specific growth rate (SGR), feed conversion (FCR), mortality and condition factor (CF) were specified from hatching to 16 th month and the results were evaluated as pre-smoltification and after-smoltification individually. Moreover, the meat yield and proximate composition of Black Sea trout’s fifth generation was specified in market size. Samplings were carried out monthly between February 2014 and May 2015. Fish were started to feed in 0.105±0.007g until they reached to 335.50±44.39g in May 2015. According to results; SGR, CF, FCR and mortality were determined as between 0.98-2.70, 0.82-1.15, 1.02-1.30 and 7.67-7.78% until smoltification stage, respectively. However, they were detected between 0.68-2.36, 1.04-1.20, 0.98-1.35 and 0.01-5.82% after smoltification. Also, the meat yield, viserosomatic index (VSI) and hepatosomatic index (HSI) were found to be 60.30%, 9.28% and 1.65% in market-size respectively. According to chemical analyses, trout meat contains crude protein as 15.47%, fat as 7.21%, ash as 1.57% and water (moisture) as 74.83%. In the view of the results, culture characteristics, proximate composition and meat yield were found similar to other cultured trout species. With these features, the fifth generation can be recommended to aquaculture sector considering the economic value of the Black Sea trout in Turkey.
TL;DR: For ideal broodstock management in Black Sea trout production, it is recommended to use 3+ and 4+ years old females with high breeding efficiency and remove 5+ years and older females from the broodstock.
Abstract: In this study, the age-dependent reproductive performance of 4th generation of hatchery-origin Black Sea trout (Salmo labrax Pallas, 1814) females was determined with the aim of understanding breeding characteristics of ideal broodstock management plan for Black Sea trout. The 2+, 3+, 4+ and 5+ years old females (n=131, 104, 63 and 28, respectively) with weights ranging from 919.45±36.22g to 2439.21±139.28g were used. Reproductive rates of females (2+, 3+, 4+ and 5+ years old) were determined as 22.14%, 60.30% and 17.56% for 2+, 17.47%, 72.82% and 9.71% for 3+, and 19.05%, 68.25%, and 12.70% for 4+ years old in November, December and January, respectively. Fertilization rates were obtained 94.31%, 95.02%, 96.03% and 93.39%, respectively. The total fecundity was positively correlated with the age and weight (r=0.84) but negatively with the relative fecundity (r=-0.24). The difference between 2+ and 3+ age groups’ total fecundities was insignificant, and the 4+ and 5+ age groups were different from all groups. In addition, there was no significant difference in fertilization rates among all age groups’ rates but the 5+ age group had slightly lower value (P<0.05). For ideal broodstock management in Black Sea trout production, based on the results of this study, it is recommended to use 3+ and 4+ years old females with high breeding efficiency and remove 5+ years and older females from the broodstock. with the first sexual maturity length of 44.76 cm in their natural environment in eastern parts of the Black Sea, they started to spawn in November and continued
TL;DR: Milt volume and sperm number were positively affected at a dose of 250 and 500 mg kg vitamin E in the diet, and a similar positive trend was also observed at the dose of 1000 mg kg.
Abstract: This study was conducted to examine the effects of feeding diets, different dose of vitamin E contains, on female and male Black Sea trout’s (Salmo labrax) reproductive performance. Broodfish were fed with dry feed contained 100 (control), 250, 500, 1000 mg kg vitamin E. Milt volume, spermatozoid number, fecundity and egg size were determined. Milt volume and sperm number were positively affected at a dose of 250 and 500 mg kg vitamin E in the diet. A similar positive trend was also observed at the dose of 1000 mg kg. Egg size is not affected by dietary vitamin E dose, but there are positive relationship between total fecundity and dietary vitamin E dose especially at the first stripping (P<0.05).