TL;DR: Allozymes provide strong confirming evidence for the hybrid origin of naturally occurring ×Ruttyruspolia because the products of specific alleles either unique to or highly characteristic of the two putative parental taxa are found combined in ×Rutanpolia.
Abstract: A plant collected in South Africa in the early 1960's has been considered an intergeneric hybrid with the parental taxa beingRuspolia hypocrateriformis (Vahl)Milne-Redhead var.australisMilne-Redhead andRuttya ovataHarv. The intermediate morphology of the plant provided the strongest evidence of its hybrid origin. The natural hybrid, named formally as ×RuttyruspoliaA. Meeuse & de Wet, is highly sterile. Crosses between the two presumed parental taxa produced two plants that are very similar to the putative natural hybrid. We had examined the presumed parental species and the natural and artificial hybrids using enzyme electrophoresis. The two parental species are highly differentiated at genes specifying soluble enzymes; they have a genetic identity of 0.51. They have no common alleles at two genes, and contain alternative alleles in very different frequencies at two loci.Ruttya andRuspolia exhibit both unique and common alleles at two additional genes. The natural and artificially produced plants of ×Ruttyruspolia are identical electrophoretically and contain alleles unique to each of the parental species at two genes. In addition, individuals of ×Ruttyruspolia combine alternative high frequency alleles from each parent at two loci. Allozymes provide strong confirming evidence for the hybrid origin of naturally occurring ×Ruttyruspolia because the products of specific alleles either unique to or highly characteristic of the two putative parental taxa are found combined in ×Ruttyruspolia.
TL;DR: The present study does not support Bremekamp's (1965) delimitation of the family Acanthaceae, involving the transfer of Lindau's (1895) subfamily Nelsonioideae to Scrophulariaceae, and the raising of his subfamilies Thunbergioidesae and MendoncioideAE to the rank of independent families.
Abstract: Structure and distribution of the foliar epidermal hairs of 109 species and two varieties belonging to 39 genera of the family Acanthaceae have been studied. Both glandular and non-glandular epidermal hairs have been recorded in the investigated taxa. The glandular hairs may be subsessile or long-stalked. The subsessile glandular hairs are of two types: i) Glandular head panduriform, 2-celled, and ii) Glandular head globular or disc-shaped, 2—8- or more-celled. Subfamilies Nelsonioideae and Thunbergioideae are characteri«*H hv thfi nanduriform hairs, while Mendoncioideae and Acanthoideae have glandular hairs with a globular head. Long-stalked glandular hairs are present only in nine species. Non-glandular hairs are also widely distributed in the family; they are present in all but ten species. They may be unicellular, or multicellular uniseriate; rarely they are branched. Though the non-glandular hairs are of diagnostic importance at species level only, in some genera like Barleria, Ruttya, , and Aphelandra, they are quite characteristic. The present study does not support Bremekamp's (1965) delimitation of the family Acanthaceae, involving the transfer of Lindau's (1895) subfamily Nelsonioideae to Scrophulariaceae, and the raising of his subfamilies Thunbergioideae and Mendoncioideae to the rank of independent families. Instead, the retention of Nelsonioideae, Thunbergioideae, Mendoncioideae, and Acanthoideae within the family Acanthaceae is favoured.