Topic
RSC complex
About: RSC complex is a research topic. Over the lifetime, 53 publications have been published within this topic receiving 4331 citations.
Papers published on a yearly basis
Papers
TL;DR: A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex and is essential for mitotic growth.
744 citations
TL;DR: Biochemical analysis revealed Rsc1 and Rsc2 in distinct complexes, defining two forms of RSC; additional genetic analysis provides further evidence that RSC function is related to transcriptional control.
236 citations
TL;DR: It is proposed that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC, a yeast remodeler RSC complex that requires their zinc cluster domain.
210 citations
TL;DR: The genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors suggest that chromatis remodeling by RSC is important for N HEJ.
Abstract: Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity. Nonhomologous end joining (NHEJ) is the major cellular repair pathway that eliminates chromosome DSBs. Here we report our genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors. Deletion of RSC30 gene or the C-terminal truncation of RSC8 impairs NHEJ of a chromosome DSB created by HO endonuclease in vivo. rsc30Delta maintains a robust level of homologous recombination and the damage-induced cell cycle checkpoints. By chromatin immunoprecipitation, we show recruitment of RSC to a chromosome DSB with kinetics congruent with its involvement in NHEJ. Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p. The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ.
193 citations
TL;DR: The results show that both prior to and following induction, specific DNA-binding proteins are the predominant determinants of chromatin architecture at the GAL1/10 genes.
193 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 3 |
| 2020 | 4 |
| 2019 | 3 |
| 2018 | 2 |
| 2015 | 3 |
| 2014 | 3 |