Topic
Ribulosephosphates
About: Ribulosephosphates is a research topic. Over the lifetime, 16 publications have been published within this topic receiving 583 citations.
Papers
TL;DR: It is concluded that strong inhibition of CO2/Mg2+ activation in the spinach enzyme is mediated by the tight binding and slow release of ribulose bisphosphate, which prevent activator CO2 and Mg2- from binding to the protein.
200 citations
TL;DR: An assay for the facile measurement of two enzymes unique to the Calvin cycle, ribulose 1,5-bisphosphate carboxylase and phosphoribulokinase, in intact cells should open the way to further studies on the regulation of CO 2 assimilation by direct measurement of the enzymes concerned within the cell.
71 citations
TL;DR: The procedure is a reliable preparative-scale method for synthesizing the dibarium salt of D-[14C]ribulose 1,5-biphosphate with a specific radioactivity up to 7 mCi/mmol and a purity near 90%.
Abstract: A procedure is described to prepare uniformly labelled D-[14C]ribulose 1,5-bisphosphate enzymically from uniformly labelled D-[14C]glucose through the coupled reactions catalysed by hexokinase (EC 2.7.1.1), glucose 6-phosphate dehydrogenase (EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 1.1.1.44) and 5-phosphoribulokinase (EC 2.7.1.19). All reagents utilized in the method are commercially available. The procedure is a reliable preparative-scale method for synthesizing the dibarium salt of D-[14C]ribulose 1,5-biphosphate with a specific radioactivity up to 7 mCi/mmol and a purity near 90%. The final product was free of other 14C-labelled sugars, sugar phosphate esters, Pi and nucleotides.
47 citations
TL;DR: In conclusion, the purified enzyme of acrylamide gels prevented the reduction of nitroblue tetrazolium in the presence of the superoxide radical, but the enzyme in solution did not react as a superoxide dismutase.
46 citations
TL;DR: From results, it appears that the ratio of carboxylase to oxygenase activity may be a variable factor with predictable subsequent alteration in the ratio between photosynthetic CO2 fixation and photorespiration.
41 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 1998 | 1 |
| 1990 | 1 |
| 1987 | 1 |
| 1986 | 1 |
| 1985 | 1 |
| 1983 | 1 |