TL;DR: In this article, the reduction/oxidation (redox) conditions in 15 principal aquifer (PA) systems of the United States, and their impact on several water quality issues, were assessed from a large data base collected by the National Water-Quality Assessment Program of the USGS.
Abstract: Reduction/oxidation (redox) conditions in 15 principal aquifer (PA) systems of the United States, and their impact on several water quality issues, were assessed from a large data base collected by the National Water-Quality Assessment Program of the USGS. The logic of these assessments was based on the observed ecological succession of electron acceptors such as dissolved oxygen, nitrate, and sulfate and threshold concentrations of these substrates needed to support active microbial metabolism. Similarly, the utilization of solid-phase electron acceptors such as Mn(IV) and Fe(III) is indicated by the production of dissolved manganese and iron. An internally consistent set of threshold concentration criteria was developed and applied to a large data set of 1692 water samples from the PAs to assess ambient redox conditions. The indicated redox conditions then were related to the occurrence of selected natural (arsenic) and anthropogenic (nitrate and volatile organic compounds) contaminants in ground water. For the natural and anthropogenic contaminants assessed in this study, considering redox conditions as defined by this framework of redox indicator species and threshold concentrations explained many water quality trends observed at a regional scale. An important finding of this study was that samples indicating mixed redox processes provide information on redox heterogeneity that is useful for assessing common water quality issues. Given the interpretive power of the redox framework and given that it is relatively inexpensive and easy to measure the chemical parameters included in the framework, those parameters should be included in routine water quality monitoring programs whenever possible.
TL;DR: Type species of 75 yeast genera examined for their ability to grow anaerobically in complex and mineral media revealed that Saccharomyces cerevisiae stands out as a yeast capable of rapid growth at low redox potentials.
Abstract: Type species of 75 yeast genera were examined for their ability to grow anaerobically in complex and mineral media. To define anaerobic conditions, we added a redox indicator, resazurin, to the media to determine low redox potentials. All strains tested were capable of fermenting glucose to ethanol in oxygen-limited shake-flask cultures, even those of species generally regarded as nonfermentative. However, only 23% of the yeast species tested grew under anaerobic conditions. A comparative study with a number of selected strains revealed that Saccharomyces cerevisiae stands out as a yeast capable of rapid growth at low redox potentials. Other yeasts, such as Torulaspora delbrueckii and Candida tropicalis, grew poorly mu max, 0.03 and 0.05 h-1, respectively) under anaerobic conditions in mineral medium supplemented with Tween 80 and ergosterol. The latter organisms grew rapidly under oxygen limitation and then displayed a high rate of alcoholic fermentation. It can be concluded that these yeasts have hitherto-unidentified oxygen requirements for growth.
TL;DR: In this article, the authors present an approach to the presentation of p/pH-Diagrams for determining the Redox potential in natural watersheds, using the Couple As(V) -As(III) as a Redox Indicator.
Abstract: Redox Potential Measurements in Natural Waters: Significance: Concepts and Problems. - Technique of Measurement, Electrode Processes and Electrode Treatment. - Characterisation of the Redox State of Aqueous Systems: Towards a Problem-Oriented Approach. - Comparison of Different Methods for Redox Potential Determination in Natural Waters. - A Novel Approach to the Presentation of p/pH-Diagrams. - The Couple As(V) - As(III) as a Redox Indicator. - In Situ Long-Term-Measurement of Redox Potential in Redoximorphic Soils. - Redox Measurements as a Qualitative Indicator of Spatial and Temporal Variability of Redox State in a Sandy Forest Soil. - Implementation of Redox Reactions in Groundwater Models. - Variance of the Redox Potential Value in Two Anoxic Groundwater Systems. - Redox Fronts in Aquifer Systems and Parameters Controlling their Dimensions. - Redox Processes Active in Denitrification. - Measurement of Redox Potentials at the Test Site 'Insel Hengsen'. - Redox Reactions, Multi-Component Stability Diagrams and Isotopic Investigations in Sulfur- and Iron-Dominated Groundwater Systems. - Redox Buffer Capacity Concept as a Tool for the Assessment of Long-Term Effects in Natural Attenuation / Intrinsic Remediation. - Redox Zones in the Plume of a Previously Operating Gas Plant. - Degradation of Organic Groundwater Contaminants: Redox Processes and EH-Values. - Microbial Metabolism of Iron Species in Freshwater Lake Sediments. - Redox Measurements in Marine Sediments. - Subject Index.
TL;DR: A label-free and simple electrochemical miRNA biosensor is developed based on employing methylene blue (MB) as a redox indicator that will be valuable for sensitive, selective and label- free detection of miRNA.
TL;DR: Novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to aThrombin is reported.