Abstract: Portulaca oleracea is a C(4) plant; however, under drought it can change its carbon fixation metabolism into a crassulacean acid metabolism (CAM)-like one. While the C(3) -CAM shift is well known, the C(4) -CAM transition has only been described in Portulaca. Here, a CAM-like metabolism was induced in P. oleracea by drought and then reversed by re-watering. Physiological and biochemical approaches were undertaken to evaluate the drought and recovery responses. In CAM-like plants, chlorophyll fluorescence parameters were transitory affected and non-radiative energy dissipation mechanisms were induced. Induction of flavonoids, betalains and antioxidant machinery may be involved in photosynthetic machinery protection. Metabolic analysis highlights a clear metabolic shift, when a CAM-like metabolism is induced and then reversed. Increases in nitrogenous compounds like free amino acids and urea, and of pinitol could contribute to withstand drought. Reciprocal variations in arginase and urease in drought-stressed and in re-watered plants suggest urea synthesis is strictly regulated. Recovery of C(4) metabolism was accounted by CO(2) assimilation pattern and malate levels. Increases in glycerol and in polyamines would be of importance of re-watered plants. Collectively, in P. oleracea multiple strategies, from induction of several metabolites to the transitory development of a CAM-like metabolism, participate to enhance its adaptation to drought.

Abstract: This study analyzed the antioxidant properties of Portulaca oleracea L., known as purslane. The samples (leaves, flowers and stems) were collected at two different locations in Portugal: Tavira (L1) and Vendas Novas (L2). Assays for total antioxidant activity, total phenolic content and ferric-reducing antioxidant power were conducted and, for both locations, significantly higher values (P < 0.05) were observed for stems (508.8 and 982.3 mg AAE/100 g DW, 1008.6 and 2285.5 mg GAE/100 g DW, 121.0 and 166.3 mg TE/100 g DW, respectively for each location), than in leaves or flowers. In the DPPH assay, the three-plant parts from L2 reached the 50% inhibition rate in lower concentrations than plants from L1. On the other hand, higher concentrations of total monomeric anthocyanins were found in samples from L1 (95.5, 88.8 and 86.0 mg/L) than in samples from L2 (81.7, 70.5 and 59.8 mg/L). The same was true for phenolic acids, estimated by liquid-chromatography, where methanol extracts were used. Phenolic extracts from all three-plant parts from both locations showed protective effects on DNA against hydroxyl radicals. This work suggests the possibility of benefit to human health from its consumption, related to the high antioxidant activity of purslane, even the stems, usually discarded in daily consumption.

Abstract: Portulaca oleracea (PO) is globally used both as a vegetable and as a herb for medical and therapeutic purposes ; hence the need to investigate its phytonutrients. The aerial parts of PO were harvested, air dried and powdered for this study. Chemical tests were carried out on the aqueous extract and the powdered specimen to determine the phytoconstituents using standard procedures. The presence of Alkaloid, saponin, tannin, flavonoid, cardiac glycoside, terpenoid, steroid, phobatannin, protein and starch were accessed qualitatively while flavonoid, tannin alkaloid and saponin were determined quantitatively and it was found not to contain steroid and phobatanin but containing 32% of saponin as its highest constituent with 26% alkaloid. This finding authenticates its use in drug production and other therapies, and to enhance further its usage and research- study. Keyword: Herb, phytoconstituents, quantitative, qualitative, screening.

Abstract: Novel small-molecule polysaccharide fractions, named POP II and POP III, were purified from Portulaca oleracea L. with average Mw values of 9.25 and 8.03 kDa, respectively. Monosaccharide analysis revealed that POP II was composed of Rha, Ara, Man, Glc, and Gal with molar ratios of 1: 1.42: 0.44: 0.88: 1.59. POP III was composed of Rha, Ara, Glc, and Gal with molar ratios of 1: 1.16: 0.23: 0.59. The antioxidant activities of the fractions were evaluated using cell-free and cell-mediated radical generating systems. POPII and POP III possessed strong antioxidant activities in both systems. The 2 novel polysaccharide fractions extracted from P. oleracea L. can be developed as natural antioxidants for treatment of free radical-related diseases.

Abstract: Portulaca oleracea referred to the common purslane considered one of the important unknown plants in Egypt. This study was carried out to investigate the biological, histopathological and anticancer effect of previous plant. Chemical composition (total acidity, protein, crude fiber, ash content, minerals, phenolic and flavonoids compounds were determined in aqueous extract of fresh plant and dried powder of portulaca oleracea. Infested rats by toxic hepatitis were feeding orally with aqueous extract compared with silymarin drug which led to prevent the increase of the serum hepatic enzyme level (ALP, AST and ALP), uric acid, nitric oxide, lipid profile and liver MDA. Antioxidant status in liver GSH were declined in rats treated with Ccl4 alone, increased after feeding orally. The histopathological examination of liver also showed that aqueous extract of portulaca oleracea reduced the incidence of liver lesions signs of hepatic toxicity and substantiates its use in various liver disorders as hepato protection. The results of anti-cancer activity showed the highest HEPG2 dead cell percentage by plant dried powder (0.547 liver cell of HEPG2) at concentration of 12.50 μg/ml. Increasing the concentration to 100 μg/ml the resulted in more higher percentage of HEPG2 dead cell (0.668) and the cytotoxic effect was determined with the Ic50 values of 17 μg/ml in HEPG2 cell line. [Hanan A. Abd El-Aziz; Sobhy M.A.; Kawkab A. Ahmed; Azza K. Abd El hamed; Zeinab A. Rahman and Wedad A. Hassan. Chemical and remedial effects of portulacaoleracea plant. Life Sci J 2014;11(6):31-42]. (ISSN:10978135). http://www.lifesciencesite.com. 5 Key word Portulacaoleracea plant, Uric acid, nitric oxide, Lipid profile, Liver MDA and antioxidant status

Abstract: Present study was formulated to find out the phytotoxic effects of different concentrations of water, ethyl acetate, petroleum ether and methanol extracts of Portulaca oleracea seeds on germination, germination index and seedlings growth of Cichorium endivia, Lactuca sativa Echinochoa crus-galli, and Brassica tournefortii. Also the total phenolics and flavonoids were determined. Results indicated that the responses of allelopathic effects were depends on extract type and concentration. Furthermore, the higher concentration had a stronger inhibitory effect on seed germination whereas in some cases the lower concentration showed a stimulatory effect. Hence, it is suggested that Portulaca oleracea seeds has strong allelopathic potential and might be use for biological control of weeds.

Abstract: Aqueous and ethanol extracts of leaf, stem and root of Portulaca oleracea L. were analyzed for phytochemical constituents and compared, whereas only aqueous extract was used for proximate and mineral composition determinations. There were presence of alkaloid, flavonoid, phenol, saponin, sterol, tannin and terpenoid in the leaf, stem and root of both extracts. Alkaloid and saponin have highest concentrations in both aqueous and ethanol extracts. High concentrations of calcium, magnesium, phosphorus and potassium were present in the leaf, stem and root, whereas iron content was least in all the parts. Level of crude protein, crude fibre and fat were highest in the leaf while carbohydrate was highest in the stem and least in the leaf at p>0.05 level of significance. Generally, it was observed that various parts of Portulaca oleracea contained different concentrations of phytochemicals and nutrients. This work, therefore, confirmed the plant’s indigenous uses. In addition, it proposed extraction of the bioactive compounds and nutrients from them for manufacture of food supplements and development of new drugs. Ethanol extract of this plant is preferable for extraction of saponin, tannin and terpenoid whereas both aqueous and ethanol extracts would produce good level of alkaloid, flavonoid, phenol and sterol.

Abstract: Objective(s): To evaluate the influences of aqueous extracts of plant parts (stem, leaves, and root) of Portulaca oleracea L. on bioformation of silver nanoparticles (AgNPs).Materials and Methods: Synthesis of silver nanoparticles by different plant part extracts of Portulaca oleracea L. was carried out and formation of nanoparticles were confirmed and evaluated using UV-Visible spectroscopy and AFM.Results:The plant extracts exposed with silver nitrate showed gradual change in color of the extract from yellow to dark brown. Different silver nanoperticles were formed using extracts of different plant parts. Conclusion: It seems that the plant parts differ in their ability to act as a reducing and capping agent.

Abstract: To compare the structural features and physicochemical properties of three species of Portulaca. Methods: Different parts of Portulaca were examined for macroscopical, microscopical characters. Physicochemical, phytochemical and fluorescence analysis of the plant material was performed according to the methods of standardization recommended by World Health Organization. Results: The plants are succulent, prostrate herbs. Usually roots at the nodes of the stem. Leaves are opposite with paracytic stomata and characteristic Kranz tissue found in C-4 plants. Abundant calcium oxalate crystals are present in all vegetative parts of the plant. Quantitative determinations like stomatal number, stomatal index and vein islet number were performed on leaf tissue. Qualitative phytochemical screening revealed the presence of alkaloids, carbohydrates, saponins, steroids and triterpenoids. Conclusions: The results of the study could be useful in setting quality parameters for the identification and preparation of a monograph.

Abstract: Background Hyperlipidemia can be cause a variety of diseases such as atherosclerosis, diabetes and fatty liver and subsequent liver enzyme increases. The Portulaca Oleracea plant has hypoglycemic and hypolipidemic properties. Therefore, in this study the effect of Portulca Oleracea herb extract on serum liver enzymes including aspartate aminotransferase (SGOT or AST), alanine aminotransferase (SGPT or ALT) and alkaline phosphatase (ALP) in rats were studied. Materials and Methods: In this experimental study, 60 Wistar rats were divided into 6 groups (n=10). Control group with normal diet, fat diet group and other groups, the experimental group received the same diet plus fat Portulaca oleracea extract maximum dose (800), the mean dose of (400), and a minimum dose of (200 mg / kg) or intraperitoneally injection (ip) and sort of Atorvastatin (10 mg kg). After the end of this period (21 days), blood sampling was performed and collected data were analyzed using the t and Tukey test, and SPSS software version 11.5. Results Comparison of statistical results indicated that alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) increase in the control group that received only fatty foods, while the experimental groups received extract of Portulaca Oleracea, and groups receiving Atorvastatin had reduced levels of

Abstract: This study was designed to evaluate the effect of 70% ethanolic crude extract of Portulaca oleracea L on mice orgons . (In vivo),In vivo, the acute toxicity of 70 % ethanolic extract of the plant on normal mice was studied. No toxic effect was noted on normal mice even at 9500 mg /kg B.W S/C injection.Histopathological changes due to ethanolic extract of the plant in healthy mice were summarized in hyperplasia of white pulp with amyloid deposition, proliferation of megakaryocytes and mononuclear cell infiltration in the liver and kidney parenchyma. There were no significant lesions detected in the brain, heart and ovary in all treated groups.

Abstract: The present investigation was carried out for evaluation of antibacterial activity of Portulaca oleracea L. against multiple drug resistant (MDR) bacteria isolated from clinical specimen. As microorganism are becoming resistant to present day antibiotics, our study focuses on antibacterial activity and future prophylactic potential of the Portulaca oleracea L. The antibacterial activity of Portulaca oleracea L. were evaluated on MDR strains such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Salmonella spp., Enterococcus faecalis, Citrobacter freundii, Acinetobacter baumannii, Streptococcus pneumoniae, Enterococcus faecium and Enterobacter cloacae. Antibacterial activity of five different solvent extracts (Methanol, acetone, ethanol, petroleum ether and n-Hexane) were prepared by using Soxhlet extractor. In-vitro antibacterial activity was performed by agar well diffusion method. The phytochemical anyalsis of leaves extract of Portulaca oleracea L. showed the presence of Saponins, Glycosides, Alkaloids, Flavonoids, Phenolic substance, Steroids, Di & Tri-terpenes and Tannins, Glycosides and Steroids. The maximum antibacterial activity of leaves of Portulaca oleracea L. found in methanolic extract. The highest zone of inhibition of methanolic leaves extract was found against E.coli (26 mm) followed by S. aureus (24 mm), S. pneumoniae (24 mm), K. pneumoniae (22 mm), S. typhi (22 mm) whereas in ethanolic extract maximum zone of inhibition was found against S. pneumoniae (22 mm), E. coli (20 mm), S. aureus (18mm), C. freundii (18 mm) and K. pneumoniae (18 mm). The lowest MIC

Abstract: Kong, Y., Rozema, E. and Zheng, Y. 2014. The effects of NaCl on calcium-deficiency disorder vary with symptom development stage and cultivar in hydroponic Portulaca oleracea L. Can. J. Plant Sci. 94: 1195–1201. Purslane (Portulaca oleracea L.) is a traditional food crop in some Mediterranean, Central American and Asian countries. Our recent study showed that this crop can be produced hydroponically in NaCl-rich nutrient solutions. Increased NaCl salinity has been found to increase the occurrence of Ca-deficiency related disorders in many horticultural crops. To explore the effects of NaCl on calcium-deficiency disorder and the associated impacts on plant growth in purslane, two commercial cultivars ‘Green’ and ‘Golden’ were hydroponically grown in nutrient solutions without Ca for the first 3 wk (Ca-deficiency period) and then in nutrient solutions with added 2.4 mM Ca(NO3)2 for 2 wk (Ca-supplement period). Two levels of NaCl (0 and 10 mM) were used in the above nutrient solutions as the two NaCl treatmen...

Abstract: Eucalyptus rostrata is known to contain various substances that are allelopathic. The allelopathic effect of leaf (ERLAE) and bark (ERBAE) of Eucalyptus rostrata aqueous extracts was evaluated for their potential uses as control on seed germination and seedling growth of two noxious weeds; Chenopodium album and Portulaca oleracea. The biological activity of the two types of extracts on germination efficiency differs with respect to the type of extract and the recipient species. Assertively, the consequence of the extracts on the two weeds was in the following order: ERLAE > ERBAE and almost, the action was highly effective and significant in C. album compared to P. oleracea. The reduction in plumule and radical lengths for the two recipient plants in the present study may be attributed to the reduced rate of cell division and cell elongation due to the presence of allelochemicals in the aqueous extracts. In conclusion, the obtained results in the present study may share in solving the problem of heavy use of synthetic herbicides via application and trying of a number of promising medicinal plants rich in allelochemicals such as E. rostrata.

Abstract: Portulaca oleracea L., an exotic weed with high medicinal value displaying typical phytoplasma associated symptoms, such as little leaf and yellowing were observed in sugarcane fields at Gorakhpur located in the state of Uttar Pradesh, India during 2013. Molecular analysis using nested-PCR with primer pairs P1/P7 and R16F2n/R16R2, evidenced that the phytoplasma found in all symptomatic plants. The nested PCR amplified product was sequenced. The approximately 1.2 kb PCR products that were observed in symptomatic Portulaca leaves had the highest nucleotide sequence similarity (99%) with members of ‘Ca. P. asteris’ group phytoplasma. Based on the BLAST analysis, the phytoplasma was classified as a member of the group 16SrI. Phylogenetic analysis also showed this phytoplasma as closely related to the reference strain for the group 16SrI. The P. oleracea phytoplasma was therefore assigned as 16SrI group and is the first report in India. Representatives of 16SrI phytoplasmas have been described in several cultivated and weed species in India and P. oleracea is an additional host species for this phytoplasma.

Abstract: Kong, Y., Rozema, E. and Zheng, Y. 2014. The effects of NaCl on calcium-deficiency disorder vary with symptom development stage and cultivar in hydroponic Portulaca oleracea L. Can. J. Plant Sci. 94: 1195-1201. Purslane (Portulaca oleracea L.) is a traditional food crop in some Mediterranean, Central American and Asian countries. Our recent study showed that this crop can be produced hydroponically in NaCl-rich nutrient solutions. Increased NaCl salinity has been found to increase the occurrence of Ca-deficiency related disorders in many horticultural crops. To explore the effects of NaCl on calcium-deficiency disorder and the associated impacts on plant growth in purslane, two commercial cultivars ‘Green’ and ‘Golden’ were hydroponically grown in nutrient solutions without Ca for the first 3 wk (Ca-deficiency period) and then in nutrient solutions with added 2.4 mM Ca(NO3)2 for 2 wk (Ca-supplement period). Two levels of NaCl (0 and 10 mM) were used in the above nutrient solutions as the two NaCl treatmen...

Abstract: In order to improve the extraction rate and quality of Portulaca oleracea seeds oil,the extraction rate and physicochemical properties of Portulaca oleracea seeds oil were selected as the indexes,ultrasound-assisted enzyme hydrolysis method was used in the pretreatment of Portulaca oleracea seeds and Soxhlet extraction method was applied on the oil extraction. The optimum conditions of enzyme hydrolysis pretreatment were determined using orthogonal trial based on single-factor experiments. gas chromatography /mass spectrometry( GC-MS) was used in the analysis of the fatty acid components of the oil extracts with the new method and compared with the tradition extraction way.The results showed that the optimum conditions of enzyme hydrolysis pretreatment were: enzyme dosage 2%,hydrolysis temperature 50 ℃,pH6. 0,liquid to solid ratio 6∶ 1. Under these conditions,the extraction rate with the combination method was up to 86. 8%,increased by 15. 43% compared to Soxhlet extraction method. The seed oils extracted by two methods both contained variety of unsaturated fatty acids. And the content of unsaturated fatty acids was over85%,content of linolenic acid was 39%.

Abstract: The present invention relates to a pharmaceutical composition for preventing or treating IL-6 mediated diseases comprising an extract of Portulaca oleracea L or a fraction thereof as an effective component, and a treatment method of IL-6 mediated diseases including a step of injecting the composition to an individual which is suspected to have IL-6 mediated diseases Also the present invention relates to a food composition and a composition for outer medical supplies for preventing or treating IL-6 mediated diseases comprising the extract of Portulaca oleracea L or the fraction thereof as an effective component

Abstract: There are many plant extracts which can modulate pain response. The aim of this study was to investigate the effects of Portulaca oleracea seed hydroalcoholic extract on pain threshold in male mice. In this laboratory experimental study, animals were randomly divided to control and groups receiving low, moderate and high doses of Portulaca oleracea seed extract. Following intraperitoneally administration of extract, pain threshold was measured using tail flick test and data were analyzed using ANOVA. Our results indicated that 6mg/kg/body weight of hydroalcoholic Portulaca oleracea grain extract has anti-stabilizing activity on RBC membrane stability in male. Increased pain threshold was observed 30 minutes and 1 hour after administration of moderate dose of extract (P<0.05 and P<0.01, respectively), and also 30 minutes and 1 hour after administration of high dose of extract (P<0.001). Our findings indicate that appropriate doses of Portulaca oleracea seed extract has pain reducing effects. E (alpha-tocopherol) and some vitamin B and carotenoids), as well as dietary minerals, such as magnesium, calcium, potassium, and iron. Also present are two types of betalain alkaloid pigments, the reddish betacyanins and the yellow betaxanthins. Both of these pigment types are potent antioxidants. The studies show that one hundred grams of fresh purslane leaves (one serving) contain about 300-400 mg of 18:3w3; 12.2 mg of alpha-tocopherol; 26.6 mg of ascorbic acid; 1.9 mg of beta-carotene; and 14.8 mg of glutathione. So, purslane is a nutritious food rich in omega-3 fatty acids and antioxidants (1)-(3). The effects of portulaca on body systems has been proved in experimental studies. The recent studies demonstrate the neuroprotective role of purslane in the striatum and proposes its prophylactic potential against developing brain damage and Parkinson's disease (4). Antitumor effect of Portulaca oleracea has also been reported (5), (6). Effects of aqueous extract of Portulaca oleracea L. on oxidative stress, liver and spleen leptin have been demonstrated (7). The aim of this study was to investigate the effects of Portulaca oleracea seed hydroalcoholic extract on pain threshold in male mice.

Abstract: The invention relates to an insecticide prepared by Portulaca oleracea The insecticide is prepared from the following components in parts by weight: 5-9 parts of Portulaca olerace extract, 55-90 parts of ethanol, 5-10 parts of tetradecane, 20-45 parts of chloroform, 1-2 parts of Celastrus angulatus, 1-5 parts of eugenol, preferentially, 2-3 parts of eugenol The insecticide can be widely applied to crops and has remarkable killing effect on insect pests; the active ingredients in the pesticide are harmless to people, livestock and poultry, practice proves that the pesticide can improve the disease resistance of crops or plants after being applied, and the insecticide is particularly suitable for killing pests of green plants in parks, streets and residential districts

Abstract: The invention discloses portulaca oleracea Linn health noodles and a preparation method thereof The health noodles comprise the raw materials such as flour, portulaca oleracea Linn, setose abelmoschus, Sedum aizoon L, kiwi fruits, russula virescens, red kojic rice, manilkara zapodilla, milk powder, Chinese chestnut powder, wax gourd, mung bean flour, coconut powder, parsley, albizia flower, fructus schizandrae, willowweed The health noodles disclosed by the invention have the beneficial effects that portulaca oleracea Linn has the effects of tonifying qi, soothing the nerves, decreasing blood pressure, enhancing human immunity, and the like, and can be eaten as a vegetable; and according to the invention, portulaca oleracea Linn and other raw materials are processed into noodles, so that the taste of the noodles is enriched, and the noodles are quick and convenient to eat, less in loss of nutrients, and high in utilization rate

Abstract: Purslane (Portulaca oleracea L.) contains polyunsaturated fatty acids (FAs) and shows anti- inflammatory, antioxidant, immunomodulatory, hypolipidemic and hypoglycemic effects, which are partly attributed to its lipid composition. Although this plant is quite abundant in Cuba, it is not usually consumed and It is any chemical study has not been informed yet. Taking into account these facts, the aim of this work was to study the FAs content of the aerial parts of purslane growing in our country. Aerial parts (leaves and stems) were collected, dried, milled in a mortar and extracted with hexane in an ultrasonic bath during 1 h. The extract was filtered and the solvent evaporated under reduced pressure in a rotary evaporator at 60 °C. A dark greenish brown liquid of oily appearance and characteristic odor was obtained with 1.7 % of yield. Samples were analyzed by gas chromatography and the FAs (1.32 % of fresh aerial parts) were determined as methyl ester derivatives. Majorities FAs were linolenic (37.83 %), palmitic (23.11 %), linoleic (14.42 %) and oleic (6.72 %), while other acids were detected in minor proportions; some of them (caprilic, capric, lauric, pentadecanoic, heptacosanoic, octacosanoic, octadecatetraenoic, gondoic and eicosatetraenoic acids) not previously detected in this species. The present study contributes to the of knowledge chemical composition of aerial parts of Portulaca oleracea L. growing in Cuba, informing the FAs content of its lipid extract.

Abstract: The invention provides portulaca oleracea L and dalbergia odorifera compound fruit and vegetable juice. The portulaca oleracea L and dalbergia odorifera compound fruit and vegetable juice is prepared from portulaca oleracea L juice, dalbergia odorifera juice and auxiliary materials. The portulaca oleracea L and dalbergia odorifera compound fruit and vegetable juice is characterized in that the mass ratio of the portulaca oleracea L juice to the dalbergia odorifera juice is 10% to 14%; the auxiliary materials comprise the following materials in percentage by mass based on the total mass: 6% of white granulated sugar, 0.12% of citric acid, 0.2% of xanthan gum, 0.01% of potassium sorbate and 0.015% of sodium isoascorbate. The portulaca oleracea L and dalbergia odorifera compound fruit and vegetable juice has the sensory indexes which are as follows: the color is light green, the mouth feel is sour and sweet and is tasty and refreshing, and cool; the juice has the special flavors of portulaca oleracea L and dalbergia odorifera; less sediment is allowable after uniform and transparent liquid is put for a long period; and physicochemical indexes are as follows: the content of soluble solids is more than or equal to 5% and the total acidity is 0.1%-0.2%.