Topic
Phthalic acid
About: Phthalic acid is a research topic. Over the lifetime, 3677 publications have been published within this topic receiving 50345 citations. The topic is also known as: o-benzenedicarboxylic acid & ortho-phthalic acid.
Papers published on a yearly basis
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Papers
TL;DR: A protocol for selective phosphopeptide enrichment using titanium dioxide (TiO2) chromatography is described, well suited for the characterization of phosphoproteins from both in vitro and in vivo studies in combination with mass spectrometry (MS).
Abstract: The characterization of phosphorylated proteins is a challenging analytical task since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically substoichiometric. Highly efficient enrichment procedures are therefore required. Here we describe a protocol for selective phosphopeptide enrichment using titanium dioxide (TiO2) chromatography. The selectivity toward phosphopeptides is obtained by loading the sample in a 2,5-dihydroxybenzoic acid (DHB) or phthalic acid solution containing acetonitrile and trifluoroacetic acid (TFA) onto a TiO2 micro-column. Although phosphopeptide enrichment can be achieved by using TFA and acetonitrile alone, the selectivity is dramatically enhanced by adding DHB or phthalic acid since these compounds, in conjunction with the low pH caused by TFA, prevent binding of nonphosphorylated peptides to TiO2. Using an alkaline solution (pH > or = 10.5) both monophosphorylated and multiphosphorylated peptides are eluted from the TiO2 beads. This highly efficient method for purification of phosphopeptides is well suited for the characterization of phosphoproteins from both in vitro and in vivo studies in combination with mass spectrometry (MS). It is a very easy and fast method. The entire protocol requires less than 15 min per sample if the buffers have been prepared in advance (not including lyophilization).
668 citations
TL;DR: In this paper, the specific adsorption of the aromatic acids, catechol, salicylic acid, benzoic acid, and phthalic acid on γ-Al2O3 is interpreted in terms of a ligand exchange model where anions of the organic acids replace the surface hydroxo groups of the Al2O 3 surface.
495 citations
TL;DR: Bi(2)WO(6) was successfully synthesized by a facile hydrothermal method, and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and BET, showing mainly square-plate-like morphologies with a short edge and the average crystalline size was in the range of 50-150 nm.
443 citations
TL;DR: In situ infrared spectroscopy has been used to investigate the adsorption of a range of simple aromatic carboxylic acids from aqueous solution to metal oxides as discussed by the authors.
346 citations
TL;DR: The data point to the existence, next to already known routes for both gram-negative and gram-positive bacteria, of alternative pathways that might be due to the presence of different dioxygenases or to a relaxed specificity of the same dIOxygenase for initial attack on polycyclic aromatic hydrocarbons.
Abstract: Cultures of Mycobacterium sp. strain PYR-1 were dosed with anthracene or phenanthrene and after 14 days of incubation had degraded 92 and 90% of the added anthracene and phenanthrene, respectively. The metabolites were extracted and identified by UV-visible light absorption, high-pressure liquid chromatography retention times, mass spectrometry, 1H and 13C nuclear magnetic resonance spectrometry, and comparison to authentic compounds and literature data. Neutral-pH ethyl acetate extracts from anthracene-incubated cells showed four metabolites, identified ascis-1,2-dihydroxy-1,2-dihydroanthracene, 6,7-benzocoumarin, 1-methoxy-2-hydroxyanthracene, and 9,10-anthraquinone. A novel anthracene ring fission product was isolated from acidified culture media and was identified as 3-(2-carboxyvinyl)naphthalene-2-carboxylic acid. 6,7-Benzocoumarin was also found in that extract. When Mycobacterium sp. strain PYR-1 was grown in the presence of phenanthrene, three neutral metabolites were identified as cis- andtrans-9,10-dihydroxy-9,10-dihydrophenanthrene andcis-3,4-dihydroxy-3,4-dihydrophenanthrene. Phenanthrene ring fission products, isolated from acid extracts, were identified as 2,2′-diphenic acid, 1-hydroxynaphthoic acid, and phthalic acid. The data point to the existence, next to already known routes for both gram-negative and gram-positive bacteria, of alternative pathways that might be due to the presence of different dioxygenases or to a relaxed specificity of the same dioxygenase for initial attack on polycyclic aromatic hydrocarbons.
345 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2026 | 1 |
| 2025 | 27 |
| 2024 | 55 |
| 2023 | 102 |
| 2022 | 222 |
| 2021 | 66 |