Topic
Phthalaldehyde
About: Phthalaldehyde is a research topic. Over the lifetime, 300 publications have been published within this topic receiving 6092 citations. The topic is also known as: OPA & OPTA.
Papers published on a yearly basis
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Papers
TL;DR: A convenient blocking reaction program using o-phthalaldehyde which can be intercalated into the sequencing format of an automated Beckman liquid-phase sequencer is presented and proved successful not only in extending sequence analyses but also in the elimination of unwanted sequences in selected peptide mixtures without the necessity of purification of the target peptide.
140 citations
TL;DR: Omicron-Phthalaldehyde reacts with primary amines to form an intensely blue-fluorescing product, and this compound is replaced with ninhydrin in this automated high-pressure liquid-chromatographic technique for determination of polyamines.
Abstract: o -Phthalaldehyde reacts with primary amines to form an intensely blue-fluorescing product. Replacing ninhydrin with this compound in our automated high-pressure liquid-chromatographic technique for determination of polyamines, we achieved a 6-to 10-fold increase in sensitivity. Linearity and reproducibility are excellent, and the method is shown to be applicable to analysis of physiological fluids and of tissue extracts.
126 citations
TL;DR: A gas chromatography-mass spectrometric method has been established for the determination of hydrazine in drinking water and surface water and the accuracy was in a range of 95-106%, and the precision of the assay was less than 13% in water.
113 citations
TL;DR: The recent introduction of fluorescamine, a novel reagent designed after the fluorogenic reaction of ninhydrin with primary amines in the presence of phenylalanine, has now provided a strong alternative to ninHydrin for detection of peptides.
Abstract: Publisher Summary The recent introduction of fluorescamine, a novel reagent designed after the fluorogenic reaction of ninhydrin with primary amines in the presence of phenylalanine, has now provided a strong alternative to ninhydrin for detection of peptides Fluorescamine reacts with primary amino groups of peptides almost instantaneously at room temperature in aqueous solution at pH 75-9, to form a fuorescent compound The reagent is subsequently hydrolyzed to yield a watersoluble, nonfluorescent product Fluorescamine itself is not fluorescent Ammonia in solution yields little or no fluorescence with fluorescamine These features and the great sensitivity attainable in the fluorescence measurement have made fiuorescamine increasingly popular in amino acid and peptide analysis Another development in recent years is the rediscovery of o -phthalaldehyde as a general reagent for amino acids and peptides when used in the presence of 2-mercaptoethanol This reagent has recently been applied to the microanalysis of amino acids and peptides
103 citations
TL;DR: Simple and sensitive methods for the separation and detection of biogenic amines by thin-layer chromatography have been developed and adapted for microradiometric enzyme assays with a high degree of sensitivity and specificity.
95 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 6 |
| 2020 | 4 |
| 2019 | 8 |
| 2018 | 2 |
| 2017 | 4 |
| 2016 | 4 |