Topic
Phosphate test
About: Phosphate test is a research topic. Over the lifetime, 19 publications have been published within this topic receiving 398 citations.
Papers
TL;DR: In this article, the authors studied the consequences of contamination of raw skim milk with Pseudomonas fluorescens CNRZ 798 on the stability of the corresponding UHT milk during storage.
Abstract: Among the incriminating factors in the destabilisation of ultra-high-temperature (UHT) milk during storage, the heat-resistant proteases of Pseudomonas are considered to play a role. The objective of this work was to study the consequences of contamination of raw skim milk with Pseudomonas fluorescens CNRZ 798 on the stability of the corresponding UHT milk during storage. After 92 days, milk destabilisation was determined by the presence of a gelled sediment and low value to phosphate test (2.4 mL for control against 0 mL of phosphate solution for milk contaminated before UHT treatment) and the presence of aggregates. For the UHT control milk and UHT milk manufactured from raw skim milk contaminated with P. fluorescens, an increase in the size of the casein micelles (205 and 332 nm, respectively), a decrease in the zeta potential (−16.6 and −14.0 mV, respectively) and decrease in the level of hydration (2.00 and 1.55 g of water per gramme of dried pellet, respectively) were detected. The increase in pH 4.6-soluble nitrogen and trichloroacetic acid-soluble nitrogen content for UHT milk previously contaminated were ten- and fivefold higher than those for control milk, respectively. The trichloroacetic acid-soluble nitrogen fraction of the milk contaminated before treatment contained 118, 22, 4 and 9 peptides from β-, αs1-, αs2- and κ-caseins in comparison to only 22, 19, 6 and 4 peptides for the control milk. This study showed that destabilisation of UHT milk was due to proteolysis of casein micelles.
49 citations
TL;DR: This study showed that destabilization of UHT milk by P. fluorescens was highly variable and strain-dependent.
48 citations
TL;DR: A gradual decrease in red blood cell loss and BARC-4 bleeding occurs with increasing platelet reactivity in patients on antiplatelet therapy undergoing CABG, and these findings support current guidelines to determine time of surgery based on an objective measurement of platelet function.
38 citations
TL;DR: The Kodak slide method and the new modified Boehringer Mannheim phosphate test were found to be interference-free, however, in some cases the latter new formulation is sensitive to substantial changes in ionic concentration of the reaction mixture.
Abstract: Phosphate concentrations were determined in 52 cases of paraproteinemia. The unmodified acidic ammonium molybdate method produced 19% spuriously high results. The false increase of phosphate concentration was attributable to formation of precipitate in the reaction mixture. The precipitate was formed by interaction between immunoglobulins and the unmodified acidic ammonium molybdate reagent. The magnitude of interference bore no relation to the type, concentrations, or isoelectric point of the paraproteins or to the presence or absence of free light chains. Diluting the sample to approximately 40 g/L total protein reduced but did not always eliminate the interference. In some cases paraprotein concentration as low as 8 g/L falsely increased plasma phosphate results. Apparently, only IgG and IgM but not IgA paraproteins produced the interference. Deproteination by ultrafiltration or by treatment with trichloroacetic acid removed the interference. The Kodak slide method and the new modified Boehringer Mannheim phosphate test were found to be interference-free. However, in some cases the latter new formulation is sensitive to substantial changes in ionic concentration of the reaction mixture.
32 citations
TL;DR: In this article, the effects of season, milking zone, process and storage conditions were highlighted, and the involved physico-chemical characteristics were determined for the region effect, numerous parameters related to the global composition and the casein micelles intervened.
Abstract: Milk samples were collected in five dairy plants located in different regions of France (North, North-West, South-West and centre of France), during spring and autumn, at receipt (bulk-raw milk), and following pasteurisation and UHT sterilisation Corresponding UHT milks were then stored at three temperatures (4, 20 and 40 °C) and analysed after different times (21, 42, 62, 90, 110 and 180 d) The physico-chemical characteristics of these different milks, including composition, micellar properties and stability as assessed by heat, ethanol and phosphate tests, were determined The database was processed by principal component analysis and common components and specific weights analysis The effects of season, milking zone, process and storage conditions were highlighted, and the involved physico-chemical characteristics were determined For the region effect, numerous parameters related to the global composition and the casein micelles intervened Some differences in milk stability as evaluated by the ethanol and phosphate tests were also observed Considering the season, spring milks had higher values of pH, lactose, soluble phosphate and micellar hydration than milks collected in autumn These spring milks also had lower values of fat and heat stability than autumn milks The UHT process effect was observed through decreases in non-casein nitrogen content and in micellar hydration and by an increase in casein micelle size for UHT milks The stability values derived from phosphate and ethanol tests were increased following the UHT process Concerning storage conditions, the temperature of 40 °C led to a decrease in pH and increases in non-casein and non-protein nitrogen contents of milks At 40 °C, low values of stability for the heat test and high values for the phosphate test were observed
29 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2020 | 2 |
| 2017 | 1 |
| 2016 | 1 |
| 2013 | 3 |
| 2012 | 1 |