Topic
Phadebas
About: Phadebas is a research topic. Over the lifetime, 78 publications have been published within this topic receiving 1291 citations.
Papers published on a yearly basis
Papers
TL;DR: It was determined that the Polilight® can be used successfully to locate saliva stains for DNA analysis and is a relatively safe, simple, noninvasive, and nondestructive technique suitable for use in forensic casework.
Abstract: Biological stains can be difficult to detect at crime scenes or on items recovered from crime scenes. The use of a versatile light source may assist in their detection. The ability of Polilight to locate potential semen, saliva, and blood stains on a range of substrates and at different dilutions was tested. We also tested the use of Polilight in comparison with conventional chemical-based presumptive screening tests such as acid phosphatase (AP), Phadebas, and luminol, often used in casework for detecting potential semen, saliva, and blood stains, respectively. The Polilight was able to locate stains that were not apparent to the naked eye. The color of the material on which a stain is deposited can have an effect on the detectibility of the stain. The Polilight was found to be comparable with the AP and Phadebas tests in terms of its sensitivity. In a comparative study between the AP test and Polilight on 40 casework exhibits, one false-negative result was observed when using the Polilight. On a series of mock casework exhibits it was determined that the Polilight can be used successfully to locate saliva stains for DNA analysis. The sensitivity of luminol for detecting potential bloodstains was greater than that of Polilight; however the Polilight has particular application in instances where a bloodstain may have been concealed with paint. Overall, the Polilight is a relatively safe, simple, noninvasive, and nondestructive technique suitable for use in forensic casework.
170 citations
TL;DR: It is demonstrated here that the RSID-saliva test can be used as a test for human salivary alpha-amylase on samples routinely examined in forensic casework and can be successfully integrated into the whole swab semen extraction method.
88 citations
TL;DR: It is concluded that saliva can be detected in the presence of blood and semen, but that high levels of amylase can occur in faecal stains.
Abstract: A method for the detection of saliva stains is described, using an insoluble substrate amylase test. The test has been used to measure the amylase activities of some biological materials apart from saliva, and it is concluded that saliva can be detected in the presence of blood and semen, but that high levels of amylase can occur in faecal stains. A screening test to locate invisble stains is also described.
71 citations
Journal Article•
TL;DR: If the results of these in vitro tests are used as the sole guide to the prescription of environmental control and immunotherapy in unselected patients with rhinitis and asthma, the performance characteristics of the CAP make it the preferred test.
Abstract: Results from three in vitro assays for allergen-specific IgE, the standard Phadebas radioallergosorbent test (PhRAST), modified RAST (mRAST), and the new Pharmacia CAP System (CAP) were compared with skin prick testing (SPT) results in 104 patients with allergic rhinitis and/or asthma and 24 nonatopic controls. Five allergens were evaluated: cat, Dermatophagoides pteronyssinus, Alternaria, June grass, and short ragweed. Using SPT results as the reference standard, the PhRAST had the lowest sensitivity (62%) and highest specificity (99%). The CAP achieved higher sensitivity (74%) with comparable specificity (96%) while the mRAST had even higher sensitivity (90%) but lower specificity (87%). The overall frequency of positive results in controls was 0% for PhRAST, 1.7% for CAP, and 3.3% for mRAST. If the threshold for a positive mRAST was raised to greater than or equal to class 2, this assay achieved performance characteristics similar to the CAP. If the results of these in vitro tests are used as the sole guide to the prescription of environmental control and immunotherapy in unselected patients with rhinitis and asthma, the performance characteristics of the CAP make it the preferred test.
66 citations
Journal Article•
TL;DR: The clinical sensitivity and specificity of an updated version of the Phadeba RAST test were superior to that of the conventional Phadebas system and an overnight first stage incubation did not contribute to an improvement in the over-all clinical effectiveness of the test system.
Abstract: The clinical sensitivity and specificity of an updated version of the Phadebas RAST test were superior to that of the conventional Phadebas system. In comparison to a three-hour first incubation, an overnight first stage incubation did not contribute to an improvement in the over-all clinical effectiveness of the test system. However, the prolonged first stage incubation did contribute to a better resolution between the normal, non-atopic control group and the atopic patient population.
56 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 2019 | 1 |
| 2018 | 2 |
| 2017 | 3 |
| 2016 | 2 |
| 2015 | 3 |