Showing papers on "Patchoulol published in 2011"
TL;DR: This simple test case of synthetic biology demonstrates that engineering the spatial organization of metabolic enzymes around a branch point has great potential for diverting flux toward a desired product.
Abstract: The ability to transfer metabolic pathways from the natural producer organisms to the well-characterized cell factory Saccharomyces cerevisiae is well documented. However, as many secondary metabolites are produced by collaborating enzymes assembled in complexes, metabolite production in yeast may be limited by the inability of the heterologous enzymes to collaborate with the native yeast enzymes. This may cause loss of intermediates by diffusion or degradation or due to conversion of the intermediate through competitive pathways. To bypass this problem, we have pursued a strategy in which key enzymes in the pathway are expressed as a physical fusion. As a model system, we have constructed several fusion protein variants in which farnesyl diphosphate synthase (FPPS) of yeast has been coupled to patchoulol synthase (PTS) of plant origin (Pogostemon cablin). Expression of the fusion proteins in S. cerevisiae increased the production of patchoulol, the main sesquiterpene produced by PTS, up to 2-fold. Moreover, we have demonstrated that the fusion strategy can be used in combination with traditional metabolic engineering to further increase the production of patchoulol. This simple test case of synthetic biology demonstrates that engineering the spatial organization of metabolic enzymes around a branch point has great potential for diverting flux toward a desired product.
216 citations
TL;DR: The sesquiterpene alcohols cis-nerolidol, α-bisabolol, cedrol, patchoulol, and santalol were screened for cytotoxicity and cytoproliferative activity on HeLa cell using Cytotoxicity Detection Kit (LDH) and the Cell Proliferation Reagent WST-1, respectively Actinomycin D and curcumin as well as the plant acids geranic and salicylic acid were used as reference samples, all known for their cytotoxic properties as mentioned in this paper.
Abstract: The sesquiterpene alcohols cis-nerolidol, α-bisabolol, cedrol, patchoulol, and santalol were screened for cytotoxicity and cytoproliferative activity on HeLa cell using Cytotoxicity Detection Kit (LDH) and the Cell Proliferation Reagent WST-1, respectively Actinomycin D and curcumin as well as the plant acids geranic and salicylic acid were used as reference samples, all known for their cytotoxic properties Among the sesquiterpene alcohols studied, cis-nerolidol possessed the strongest cytotoxic properties (165 μM ± 67) Low cytotoxicity and influence on HeLa cell viability was demonstrated for geranic and especially salicylic acid
15 citations
Patent•
5 May 2011
TL;DR: In this paper, a method of producing patchoulol and 7-epi-α-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp) was proposed.
Abstract: A method of producing patchoulol and 7-epi-α-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). The method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery.
3 citations
Patent•
5 May 2011
TL;DR: In this paper, a method of producing patchoulol and 7-epi-α-selinene, which can be useful in the field of perfumery, is described.
Abstract: The present invention provides a method of producing patchoulol and 7-epi-α-selinene, said method comprising contacting at least one polypeptide with farnesyl phyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing patchoulol and 7-epi-α-selinene is also an object of the present invention.
TL;DR: Biochemical variables differed significantly among the treatments, with the exception of total sugar levels, and the highest concentrations of total sugars were observed in the calli and in vitro-grown plantlets of POG014 and POG021.
Abstract: Patchouli is an aromatic shrub of commercial interest because its essential oil is rich in patchoulol. This study aimed to evaluate the effect of growth regulators on callus production, analyze the essential oil production in calli and evaluate metabolic differences between callus, in vitro grown-plantlets and greenhouse-grown plants in three different accessions of patchouli. Calli were induced from leaf explants on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 6-benzyladenine (BA). The largest size calli from different accessions were obtained in the presence of the two plant growth regulators (PGRs). For accession POG014, presence of 0.022 mg l−1 2,4-D plus 0.022 mg l−1 BA were optimum. For accession POG021, presence of 0.110 mg l−1 2,4-D plus 0.022 mg l−1 of BA induced the largest callus, whereas for accession POG002, 0.022 mg l−1 2,4-D and 0.225 mg l−1 BA, as well as 0.11 mg l−1 2,4-D and 0.022 mg l−1 BA promoted the development of largest callus. Among all accessions, peroxidase activity was highest in organogenic calli of accession POG014, whereas, polyphenol oxidase activity was highest in in vitro-grown plantlets of accession POG021. Biochemical variables differed significantly among the treatments, with the exception of total sugar levels. The highest concentrations of total sugars were observed in the calli and in vitro-grown plantlets of POG014 and POG021. Essential oils were not detected in callus tissues.
TL;DR: In this paper, four new patchoulol derivatives, 8 alpha, 9 alpha-dihydroxypatchoulol (1), 3 alpha,8 alpha-Dihd-RH, 6 alpha-HOG, and 2 beta,12-DRH, were isolated from the aerial part of Pogostemon cablin (Labiatae), together with nine known compounds, sesquiterpenoids 5-8 and flavonoids 9-13.
Abstract: Four new patchoulol derivatives, 8 alpha,9 alpha-dihydroxypatchoulol (1), 3 alpha,8 alpha-dihydroxypatchoulol (2), 6 alpha-hydroxypatchoulol (3), and 2 beta,12-dihydroxypatchoulol (4), were isolated from the aerial part of Pogostemon cablin (Labiatae), together with nine known compounds, sesquiterpenoids 5-8 and flavonoids 9-13. Their structures were elucidated by detailed spectroscopic analysis, using 1D- and 2D-NMR techniques.