TL;DR: The solubility of the sesquiterpene alcohol patchoulol in supercritical carbon dioxide was measured at P ranging from 10.0 MPa to 25.0MPa and T of 40.0 and 50.0 °C using a simple microsampling type apparatus with a 100.5 µL sample loop for off-line analysis.
Abstract: The solubility of the sesquiterpene alcohol patchoulol in supercritical carbon dioxide was measured at pressures (P) ranging from 10.0 MPa to 25.0 MPa and temperatures (T) of 40.0 and 50.0 °C using a simple microsampling-type apparatus with a 100.5 μL sample loop to remove aliquots for off-line analysis. The system was first validated using vanillin with off-line spectrophotometric analysis, then utilized for patchoulol measurements with off-line GC−MS analysis. The measured solubility of patchoulol in supercritical CO2 ranged from mole fractions of 0.43 × 10-3 at 10.0 MPa and 50.0 °C to mole fractions of 9.4 × 10-3 at 25.0 MPa and 40.0 °C.
TL;DR: It was observed that the oil yield decreased gradually with the increase in weed biomass, but the percentage of patchoulol showed a different behaviour, which may decrease its commercial value.
TL;DR: In this paper, a method for increasing the patchoulol content in essential-oil extracted from leaves of Pogostemon cablin is provided, comprising contacting a dried patchouli biomass with microbial cultures, incubating the patchioli biomass under conditions suitable for allowing the increase in the patcholol content and extracting the oil.
Abstract: A process is provided for improving sensory properties of essential oils by treatment and incubation with a microbial culture during the extraction process. A method for increasing the patchoulol content in essential- oil extracted from leaves of Pogostemon cablin is provided, comprising contacting a dried patchouli biomass with microbial cultures, incubating the patchouli biomass under conditions suitable for allowing the increase in the patchoulol content and extracting the oil. This process results in increased patchoulol content without decreasing the overall oil yields. The patchouli oil also has enriched aroma.