Topic
Partial cloning
About: Partial cloning is a research topic. Over the lifetime, 16 publications have been published within this topic receiving 550 citations. The topic is also known as: PCL.
Papers
TL;DR: The data indicate that CD34 would, similar to its role in the hematopoietic system, be an important probe for characterizing the cellular biology of the hepatic stem‐cell compartment.
139 citations
TL;DR: Results from the recent cDNA analysis of heparan-sulfate 2-solfotransferase suggest that at least two different gene products are responsible for 6- and 2-O-Sulfations ofheparan sulfate.
117 citations
TL;DR: The Slc12a2 gene encodes a widely expressed bumetanide-sensitive Na+-K+-2Cl-cotransporter that participates in various functions such as Cl- secretion and cell volume regulation and the identification of an alternatively spliced variant of the cotransporter, expressed primarily in brain.
Abstract: The Slc12a2 gene encodes a widely expressed bumetanide-sensitive Na+-K+-2Cl−cotransporter that participates in various functions such as Cl− secretion and cell volume regulation. We isolated and ch...
92 citations
TL;DR: A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis, was used to study morphological and biochemical responses to 20-hydroxyecdysone, demonstrating the usefulness of this cell line for the study of ecdysone response and the isolation of the receptor components in A. grandis.
Abstract: A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis (BRL-AG-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20E). The cells respond to 10(-6) M 20E by inhibition of cell growth and enhanced production of some secreted proteins. Crude nuclear extracts containing the ecdysteroid receptor complex proteins consisting of the ecdysteroid receptor (EcR) and ultraspiracle (USP) bound ponasterone A with a Kd of 6.1 nM. Bound radiolabeled ponasterone A was displaced by both 20E and the lepidopteran-specific non-steroidal ecdysteroid agonist, RH-5992, with 41- and about 1,900-fold higher Kd values, respectively. We identified the ecdysteroid receptor components in this cell line, using monoclonal antibodies against the Drosophila ecdysteroid receptor (DmEcR) and ultraspiracle (DmUSP) proteins. A predominant band of about 70 kDa was-detected with anti-EcR, and multiple bands ranging from 50-55 kDa were detected with anti-USP in the A. grandis extracts. Using degenerate primer RT-PCR, we isolated a 450 bp cDNA fragment of the putative AgEcR. Using this fragment as a probe, we identified a large mRNA of ca. 10 kb by Northern blot analysis. These results demonstrate the usefulness of this cell line for the study of ecdysone response and the isolation of the receptor components in A. grandis.
38 citations
TL;DR: In this article, a mouse kNBC-3 full-length cDNA was cloned from inner medullary collecting duct (mIMCD-3) cells and the tissue distribution and functional properties of NBC-3 were determined using established methods.
38 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2015 | 1 |
| 2008 | 1 |
| 2004 | 1 |
| 2001 | 1 |
| 2000 | 1 |
| 1998 | 1 |