Topic
Paraformaldehyde
About: Paraformaldehyde is a research topic. Over the lifetime, 951 publications have been published within this topic receiving 19121 citations.
Papers published on a yearly basis
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Papers
TL;DR: Using this fixative and the peroxidase-labeled antibody technique, basement membrane antigen was localized within the cisternae of endoplasmic reticulum of parietal yolk sac cells and in extracellular basement membranes with adequate tissue preservation, a task which has not been successfully accomplished by conventional fixatives.
Abstract: A new fixative which primarily stabilizes carbohydrate moieties was developed for immunoelectron microscopy. It contains periodate, lysine and paraformaldehyde. Theoretically, the carbohydrates are oxidized by periodate and cross-linked by lysine. The fixative can preserve antigenicity as well as paraformaldehyde and ultrastructure as well as glutaraldehyde. Using this fixative and the peroxidase-labeled antibody technique, basement membrane antigen was localized within the cisternae of endoplasmic reticulum of parietal yolk sac cells and in extracellular basement membranes with adequate tissue successfully accomplished by conventional
4,024 citations
TL;DR: The buffered picric acid paraformaldehyde fixative has been supplemented with glutaraldehyde and used as primary fixative for the perfusion of rat brains and it is suggested that the procedure may be useful for electron-microscopic sampling of immunoreactive structures occurring infrequently over a large area or for the electron- microscopically classified neurons.
584 citations
TL;DR: Aldehydes are the most commonly used fixative for microscopy and serve to stabilize the fine structural details of cells and tissues prior to examination by light or electron microscopy.
Abstract: Aldehydes are the most commonly used fixatives. They serve to stabilize the fine structural details of cells and tissues prior to examination by light or electron microscopy. Research workers, technicians, pathologists and others who regularly use aldehyde fixatives frequently do not appreciate the nature and properties of these compounds or the reasons for choosing to fix a specimen in formaldehyde, glutaraldehyde or a mixture of the two. Misconceptions are widespread also about formalin and paraformaldehyde, the commercial products from which formaldehyde-containing solutions are made.
535 citations
TL;DR: The use of in situ hybridization offers a highly sensitive method to study cytokine production in individual cells, but the technqiue is fairly time-consuming and the presence of cytokine mRNA does not guarantee that it will be translated.
Abstract: Cytokine production needs to be evaluated by multiple approaches since all existing methods for quantification are hampered by various drawbacks. Bioassays measure functional properties, but they are rarely monospecific and the results are influenced by inhibitory factors. Techniques based on ELISA and RIA systems are highly specific but do not distinguish between biologically active and inactive substances. Furthennore, these assays determine the extracellular presence of a secreted interieukin, reflecting the net outcome of produced, absorbed and degraded cytokine. The demonstration of a receptor-directed focusing of lymphokine release by helper T cells (Poo et al. 1988) argues for direct cellular approach to cytokine assessment. The use of in situ hybridization offers a highly sensitive method to study cytokine production in individual cells, but the technqiue is fairly time-consuming and the presence of cytokine mRNA does not guarantee that it will be translated.
479 citations
TL;DR: It is suggested that during fixation most NADPH-diaphorase activity is inactivated and only some of the NAD PH- diaphorases activity associated with soluble nitric oxide synthase remains intact.
444 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 11 |
| 2024 | 28 |
| 2023 | 57 |
| 2022 | 75 |
| 2021 | 19 |
| 2020 | 20 |