TL;DR: This study used sequence data of two mitochondrial genes and one nuclear gene to resolve the phylogeny of a closely related subgroup of the Trochidae, 30 species of largely Southern Hemisphere monodontine topshells, allowing for the first time assignation (based on DNA sequence) of clearly defined, well-supported taxonomic and nomenclatural classified species.
TL;DR: This analysis delimits five molecular operational taxonomic units (MOTUs), and distinguishes O. impervia from O. variegata, revealing important discrepancies between MOTUs and morphology-based species identification and discussing alternative hypotheses that can account for this.
Abstract: The genus Oxystele, a member of the highly diverse marine gastropod superfamily Trochoidea, is endemic to southern Africa. Members of the genus include some of the most abundant molluscs on southern African shores and are important components of littoral biodiversity in rocky intertidal habitats. Species delimitation within the genus is still controversial, especially regarding the complex O. impervia / O. variegata. Here, we assessed species boundaries within the genus using DNA barcoding and phylogenetic tree reconstruction. We analysed 56 specimens using the mitochondrial gene COI. Our analysis delimits five molecular operational taxonomic units (MOTUs), and distinguishes O. impervia from O. variegata. However, we reveal important discrepancies between MOTUs and morphology-based species identification and discuss alternative hypotheses that can account for this. Finally, we indicate the need for future study that includes additional genes, and the combination of both morphology and genetic techniques (e.g. AFLP or microsatellites) to get deeper insight into species delimitation within the genus.
TL;DR: It is revealed that Australian and New Zealand species fall into endemic clades that have been separated for, at most, 35million years and this divergence date postdates a vicariant split by around 50million years, suggesting that, once again, long-distance trans-Tasman dispersal has played a pivotal role in molluscan evolution in this part of the world.