Osteochilus

Abstract: . Kurniawan A, Hariati AM, Kurniawan A, Wiadnya DGR. 2021. First genetic record and the phylogenetic relationship of Osteochilus spilurus (Cyprinidae: Labeoninae) originating from Bangka and Belitung Islands, Indonesia. Biodiversitas 22: 794-802. Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fish in Indonesia is still solely limited to morphological characteristics. Therefore it is highly important to validate using short DNA sequences that have been proven to be fast, effective, and accurate. Fish specimens were collected from Lenggang river in Belitung island, and Lebak river in Bangka island, Indonesia. Mitochondrial cytochrome b gene (cyt b, 408 bp) was amplified and sequenced. We collected fish specimens from Lenggang River in Belitung Island and Lebak River in Bangka Island, Indonesia. The results suggested that individuals from both islands are clustered together with a strong bootstrap value (100%) in a monophyletic clade. The specimens deposited at NCBI as the first Indonesian genetic records. They have a sister lineage to individuals from Sabah, Malaysia, although supported by a low bootstrap value. While geographical connection during the Pleistocene period allowed wide distribution of the species, differences in the evolutionary processes of historical biogeography and environmental changes are the predicted cause of the low genetic similarity. These findings can be used as a sequence reference and basic information for conservation of this species.

Abstract: A study on kidney histology of Silver Sharkminnow ( Osteochilus hassletii ) sampled from Maninjau and Singkarak Lakes, West Sumatra has been done from December 2012 to April 2013 at Animal Structure and Development Laboratory, Department of Biology, Andalas University, Padang. Direct observation and purposive sampling were conducted at four locations around Singkarak Lake (Sumani, Paninggahan, Sumpur, Ombilin) and one location in Maninjau Lake (Tanjung Sani). Histological samples of kidneys were isolated, fixated, dehydrated and embedded on paraffin and stained using Haematoxylin-Eosin. The results showed some histological alterations of kidney samples from all locations. Those alteration varied from cell hypertrophy, lysis, necrosis and emerging of scrape tissues. There were no significant differences on the degree of alteration among all populations. Those result indicated that both lakes had been experienced organic pollutions. Keywords: Silver Sharkminnow ( Osteochilus hasseltii C.V), Histopatology, Kidney, Singkarak Lake, Maninjau Lake.

Abstract: Osteochilus spilurus is one of freshwater fish in the river of Bangka and Belitung Island. This fish became an important economically fish in East Belitung. The problem of Osteochilus spilurusis is the season of fishing and trading which may contribute to reduce its natural population. Nowadays, Osteochilus spilurus is still at the first level of domestication. Adaptation using recirculation of water in an artificial container needs to test to pass the first level of the domestication process. Different debit of water recirculation affects survival rate but does not at the growth of Osteochilus spilurus. The best treatment was with water debit of 0.11 liters/minute, resulted in a survival rate of 88.33 ± 0.03%, length growth of 0.31 ± 0.06 cm and weight growth of 0.93 ± 0.04 g for 30 days of cultivation. Aggressiveness and sensitivity of Osteochilus spilurus decrease after 7 days of cultivation. The researcher can use water circulation in the next level of Osteochilus spilurus domestication.

Abstract: Background: Kelabau ( Osteochilus spp.) is a freshwater fish commonly found in the rivers of Riau, Indonesia. Researchers believe that these are Osteochilus kelabau; however, accurate taxonomic determination of these fish in Riau waters has not been made. The purpose of this study was to facilitate the identification of the kelabau based on its morphology and genetics using biometric and cytochrome c oxidase subunit 1 ( CO1) analyses, respectively. Methods: Fish samples were collected from the Siak, Kampar and Rokan rivers in Riau Province, Indonesia. The DNA of 90 fish was extracted from the caudal fins using a DNA extraction kit, after which it was amplified using primers Fish-F1 and Fish-R1. Sequencing was conducted by Applied Biosystems Macrogen Korea, and the DNA sequences were then edited and aligned using MEGA v. 7. All samples were BLAST-searched for identification using the National Center for Biotechnology Information and BOLD System. Phylogenetic trees were constructed, and the similarity index was calculated using accession numbers AP011385.1 and KC631202.1 in GenBank. Results: Analysis of the consensus barcode sequence for 86 species revealed a high percentage of barcode matches (96%-97% in GenBank and 96.6%-96.76% in the BOLD System). The nucleotide distance between groups of kelabau from the different rivers based on the Kimura 2-parameter model gave the following results: 0.05% between groups from the Siak and Kampar rivers, 0.09% between those from the Siak and Rokan rivers and 0.05% between those from the Kampar and Rokan rivers. The nucleotide distance between the groups in the Siak (0.09%), Kampar (0.00%) and Rokan (0.10%) Rivers indicated that the kelabau in those rivers were related to each other. Conclusions: Based on the results of the research data using CO1 and biometric analyses, the kelabau were confirmed to be O. melanopleurus.