TL;DR: It is shown that oskar RNA is localized to the posterior pole of the oocyte when germ plasm forms and proposed that the pole plasm is assembled stepwise and that continued interaction among its components is required for germ cell determination.
TL;DR: The oskar gene directs germ plasm assembly and controls the number of germ cell precursors formed at the posterior pole of the Drosophila embryo.
Abstract: The oskar gene directs germ plasm assembly and controls the number of germ cell precursors formed at the posterior pole of the Drosophila embryo. Mislocalization of oskar RNA to the anterior pole leads to induction of germ cells at the anterior. Of the eight genes necessary for germ cell formation at the posterior, only three, oskar, vasa and tudor, are essential at an ectopic site.