Topic
Olfactory Supporting Cell
About: Olfactory Supporting Cell is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 114 citations.
Papers
TL;DR: Analysis of the data for correlation between basal and supporting cell proliferation revealed a weak correlation in neonates but no correlation in older animals, suggests that the mechanisms that regulate proliferation of the two cell types are different, and indicates that the proliferation of supporting cells is related to growth in surface area of the epithelium.
Abstract: It is well known that progenitor cells in the basal layer of olfactory epithelium proliferate continuously throughout life; the offspring of these dividing cells produce replacements for receptor neurons In the rat the number of proliferating basal cells/mm length of epithelium (proliferation density) decreases with postnatal age while the area of the olfactory sheet increases The supporting cells, which act as the glia of the olfactory epithelium, also divide We examined in detail some aspects of the dynamics of olfactory supporting cell proliferation to determine whether their rate of proliferation changes with age, and how it compares with the rate in basal progenitor cells Using BrdU to label dividing cells, we determined the proliferation density of supporting cells and basal cells in 10 μm coronal sections from six different anterior-posterior regions in rats ranging in age from birth (P1) until P333 We observed a dramatic decrease in supporting cell proliferation density from P1 (80 cells/mm) to P11 (32 cells/mm) to P21 (12 cells/mm); the density decreases continuously to P333 (04 cells/mm) This reduction was even more dramatic than that in the basal cell population (Weiler and Farbman, 1997) Analysis of the data for correlation between basal and supporting cell proliferation revealed a weak correlation in neonates but no correlation in older animals This suggests that the mechanisms that regulate proliferation of the two cell types are different Our data also indicate that the proliferation of supporting cells is related only to growth in surface area of the epithelium No turnover seems to occur in the supporting cells as it does in the olfactory neurons, where proliferation of basal cells is necessary for both growth and replacement GLIA 22:315–328, 1998 © 1998 Wiley-Liss, Inc
63 citations
TL;DR: A considerable fraction of the WGA‐bound particles could reflect transmembrane proteins in olfactory dendritic endings and cilia and in respiratory cilia.
Abstract: Binding of colloidal gold-conjugated lectins was studied in cilia and microvilli of rat olfactory and respiratory epithelia. This was done in sections of rapidly frozen, freeze-substituted specimens embedded in Lowicryl K11M or, for wheat germ agglutinin (WGA) alone, in deep-etched replicas. Olfactory dendritic endings and cilia labeled with WGA and faintly with soybean agglutinin (SBA); olfactory supporting cell microvilli bound only Dolichos biflorus agglutinin (DBA). Microvilli of an infrequent cell bound peanut agglutinin (PNA), SBA, and WGA. These microvilli labeled more strongly with the last two lectins than the olfactory cilia. Respiratory cilia bound WGA and, somewhat more weakly, PNA; microvilli of ciliated respiratory cells bound all four lectins. Visualization of specific labeling improved after preincubation of sections with neuraminidase, except for DBA where lectin binding was abolished. PNA labeling was seen only after neuraminidase preincubation. The densities of membrane surface particles that labeled with WGA corresponded with those of fracture plane particles in a quantitative freeze-fracture, deep-etch analysis. Therefore, a considerable fraction of the WGA-bound particles could reflect transmembrane proteins in olfactory dendritic endings and cilia and in respiratory cilia. The possible nature of these particles is discussed.
43 citations
Journal Article•
TL;DR: Preliminary cytochemistry on olfactory and nasal respiratory epithelial samples with the lectin Concanavalin A (Con A) and antibodies against Olfactory marker- and odorant-binding proteins and, in taste only, the sweet-tasting protein thaumatin, were sufficiently successful to warrant further endeavors.
Abstract: Rat olfactory and respiratory epithelia and Rhesus monkey taste buds were studied with rapid-freeze, acetone/0.1% uranyl acetate freeze-substitution and low-temperature Lowicryl K11M embedding, usually in the absence of other chemical fixation and cryoprotection procedures. Ultrastructural features of mucus, cytoplasm, including cytoskeletons, and membranes were better retained than with conventional methods. Some major examples: The mucus of the olfactory epithelium consisted of a single layer; that of the respiratory epithelium had an electron-opaque sol layer surrounding cilia and microvilli below a thin laminated electron-lucent gel layer. Taste-bud pores displayed a foam-like opaque secretory product, resembling the contents of secretory granules within Type I taste-bud cells. The electron-opacity of cytoplasmic matrices sometimes obscured features such as radial spokes of respiratory cilia. Membranes had smooth outlines; those of olfactory receptor cell cilia were more electron-opaque than those of olfactory supporting cell microvilli and respiratory cilia. Membranous monolayers of many respiratory cilia across large arrays often partially split apart, all in the same direction. The space between those monolayers contained an electron-lucent substance. Preliminary cytochemistry on olfactory and nasal respiratory epithelial samples with the lectin Concanavalin A (Con A) and antibodies against olfactory marker- and odorant-binding proteins and, in taste only, the sweet-tasting protein thaumatin, were sufficiently successful to warrant further endeavors.
18 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 1998 | 1 |
| 1992 | 1 |
| 1989 | 1 |