TL;DR: The results indicate that vegetative chromosome doubling of selected diploid clone generally will not produce high yielding tetraploid clones and that these materials will be very useful in breeding and for genetic investigations.
Abstract: The performance of 15 diploid clones and their vegetatively doubled counterparts was evaluated in the field for 2 years. The diploid clones produced more stems and were taller 4 weeks after planting, flowered earlier, and produced more total tuber yield. The significantly greater total tuber production of the diploids was due mainly to having more tubers less than 2.5 inches in diameter. The tetraploid clones usually produced more and heavier tubers over 2.5 inches in diameter, but not enough to offset the higher total tuber number of the diploids. Male and female fertility were evaluated in the greenhouse and although tetraploid seed set was 50% less than for the diploids, the doubled clones were sufficiently fertile for use in genetic and breeding programs. The results indicate that vegetative chromosome doubling of selected diploid clones generally will not produce high yielding tetraploid clones. These materials will be very useful in breeding and for genetic investigations.
TL;DR: The results demonstrate that offset planting programs can be hampered by low amenability of plant species to propagation but that identifying a successful propagation method can lead to rapid gains in plant production.
Abstract: The Kogan waxflower (Philotheca sporadica) is a threatened and geographically restricted plant species that is being impacted by gas pipeline construction. This study assessed seed, cutting and tissue culture methods for propagation of P. sporadica for an environmental offset program. Fresh seed could not be germinated under nursery conditions and less than 1.5% of cuttings produced roots. However, in vitro proliferation of shoots in tissue culture media containing 4.4 μM benzyladenine provided over 5,000 shoots from each initiated shoot tip in a 6 month period. Shoots were converted readily into plantlets using 314.9 μM indole-3-butyric acid for root induction and an in vitro soil-less system for transferring plantlets from the laboratory to the nursery. These results demonstrate that offset planting programs can be hampered by low amenability of plant species to propagation but that identifying a successful propagation method can lead to rapid gains in plant production.
TL;DR: In this paper, the authors describe the use of clonal propagation techniques for an offset program that propagated two species impacted by a landslide and road works, and highlight challenges and successes in propagating poorly-known species for an environmental offset program within a short timeframe and with little prior knowledge of suitable propagation methods.
Abstract: Background: Environmental offset programs for threatened plant species can be limited by the capacity to propagate sufficient plants for establishing offset plantations. This study describes the use of clonal propagation techniques for an offset program that propagated two species impacted by a landslide and road works. Materials and Methods: Cuttings were collected from Pomaderris clivicola (Rhamnaceae) and Bertya pedicellata (Euphorbiaceae) on 9 and 7 occasions, respectively. Shoots were also initiated into tissue culture. Results: Both species proved extremely difficult to propagate from cuttings and tissue culture. Rooting frequencies for P. clivicola cuttings were 4.3%. Repeated harvests of cuttings from the impacted plant population over more than 2 years eventually provided most of the P. clivicola plants required for the offset planting. However, the offset population of P. clivicola also had to be supplemented with some plants produced by tissue culture and with one plant that was excavated from the impacted population. Rooting frequencies for B. pedicellata cuttings were only 1.3%. A combination of cutting propagation and tissue culture did not produce sufficient B. pedicellata plants for the offset planting, but the offset population was supplemented with 27 plants that were excavated during road works. Conclusion: Success in producing the offset plants depended ultimately on a combination of (a) Cutting propagation, (b) Tissue culture propagation and (c) Whole plant excavation and translocation. This case study highlights challenges and successes in propagating poorly-known species for an environmental offset program within a short timeframe and with little prior knowledge of suitable propagation methods.; ;
TL;DR: A method for increasing the survival rate of collection and preservation of wild and affinis banana germplasm resources is proposed in this article, where the method comprises the steps of digging of offset buds, transportation and preservation, field planting of the offset buds and establishment of a bionic environment.
Abstract: The invention discloses a method for increasing the survival rate of collection and preservation of wild and affinis banana germplasm resources. The method comprises the steps of digging of offset buds, transportation and preservation of the offset buds, field planting of the offset buds and establishment of a bionic environment. The effective cultivation method is formed in the existing aspects of digging of the offset buds, transportation and preservation of the offset buds, field planting of the offset buds and establishment of the bionic environment, the survival rate is at least increasedto 90% or above, and the collected rare wild banana resources are effectively preserved.
TL;DR: Buddleia 'Glass Slipper' is a short, mounded, multi-stemmed, winter-hardy butterfly bush plant with long, compact, self-cleaning flowering thyrse over a long season beginning mid-summer with sweetlyfragrant pale periwinkle flowers that are attractively offset by silvery green foliage as mentioned in this paper.
Abstract: Buddleia ‘Glass Slipper’ is a short, mounded, multi-stemmed, winter-hardy butterfly bush plant with long, compact, self-cleaning flowering thyrse over a long season beginning mid-summer with sweetly-fragrant pale periwinkle flowers that are attractively offset by silvery green foliage.