Showing papers on "Nanoparticle tracking analysis published in 2011"
TL;DR: By combining NTA with fluorescence measurement it is demonstrated that vesicles can be labeled with specific antibody-conjugated quantum dots, allowing their phenotype to be determined, demonstrating that NTA is far more sensitive than conventional flow cytometry.
1,313 citations
TL;DR: The size of the exosomes decreased at 4°C and 37°C, indicating a structural change or degradation, and multiple ultracentrifugation also did not change theExosome size.
804 citations
TL;DR: In this paper, the size distributions of commercially produced near spherical nanoparticles have been determined using both single particle (transmission electron and atomic force microscopy) and ensemble methods (dynamic light scattering and nanoparticle tracking).
155 citations
TL;DR: In this article, the authors present a characterization system for nanoparticles at the nanoscale using the Nanoparticle tracking analysis (NPSA) technology. But their focus is on the characterization of particles at the nano-scale.
62 citations
TL;DR: In this article, the authors investigate the precipitation of a poorly water soluble drug (tolnaftate) from low, mu M concentration solutions, using the applicability of nanoparticle tracking analysis (NTA; the NanoSight instrument), with comparison to results from dynamic light scattering (DLS).
16 citations
TL;DR: The Nanoparticle Tracking Analysis technique is demonstrated that sizes nanoparticles in suspension, based on their Brownian motion, and can be used to complement existing techniques for the sizing of nanoparticles (e.g., DLS, PCS) allowing data obtained from these methods to be validated by direct microscopical observation of the sample.
Abstract: The assessment of the complete distribution of nanoparticle sizes within a suspension is notoriously difficult to carry out. We demonstrate the Nanoparticle Tracking Analysis (NTA) technique that sizes nanoparticles in suspension, based on their Brownian motion. This technique has found significant use in the field of nano- and eco-toxicology, in several research groups showing of the technique to assess a range of engineered nanoparticles including gold, SiO2, TiO2 and polystyrene. This capability shares many features in common with conventional flow cytometry but is unique in this deeply sub-micron size range. NTA is a direct and fast technique by which nanoparticles in their natural solvated state in a liquid can be rapidly detected, sized and counted. The technique can be used to complement existing techniques for the sizing of nanoparticles (e.g., DLS, PCS) allowing data obtained from these methods to be validated by direct microscopical observation of the sample.
9 citations
1 Jan 2011
TL;DR: The possibility of rapidly monitoring the presence and relative quantity of exosomes in both tissue culture supernatants and body fluids by the technique of nanoparticle tracking analysis (NTA) may represent a significant step forward in the characterisation of exOSomes.
Abstract: Many cells, including those of the immune system, secrete small vesicles called exo somes or nanovesicles, with a size range of between 50 and 150 nm. These vesicles can display a wide range of immunological func tions, including both immunostimulatory and immunosuppressive activities. The pos sibility of rapidly monitoring the presence and relative quantity of exosomes in both tissue culture supernatants and body fluids by the technique of nanoparticle tracking analysis (NTA) may represent a significant step forward in the characterisation of exosomes.
2 citations