Showing papers on "Naja published in 1973"
TL;DR: Six phospholipase A-containing fractions were isolated from cobra venom by ion-exchange and gel filtration chromatography and exhibited similar specific activities.
36 citations
TL;DR: Two neurotoxins were purified from Naja nigricollis venom using equilibrium chromatography and the usefulness of N-terminal automatic sequencing for rapid identification of proteins is discussed.
Abstract: Two neurotoxins were purified from Naja nigricollis venom using equilibrium chromatography. This method was shown to produce the most resolution to purify proteins having very close physico-chemical properties. The toxins obtained were characterized, as well as three neurotoxins previously isolated from Naja haje venom, by the determination of N-terminal (35 to 39 residues) and C-terminal sequences.
The usefulness of N-terminal automatic sequencing for rapid identification of proteins is discussed.
24 citations
TL;DR: The binding of small amounts of DLF to intact red cells, observed by bioassay, was due to the presence of a small fraction of lysed cells, and it is concluded that the binding to ghosts is not related to the lytic effect ofDLF but secondary to lysis.
Abstract: The binding of direct lytic factor (DLF) from cobra venom (Naja naja) to intact guinea-pig red cells and to guinea-pig ghosts was estimated quantitatively by bioassay of DLF in the supernatant.
10 citations