Topic
Mycosphaerella eumusae
About: Mycosphaerella eumusae is a research topic. Over the lifetime, 20 publications have been published within this topic receiving 373 citations.
Papers
TL;DR: In this paper, the authors collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set.
Abstract: Three species of Mycosphaerella, namely M. eumusae, M. fijiensis, and M. musicola are involved in the Sigatoka disease complex of bananas. Besides these three primary pathogens, several additional species of Mycosphaerella or their anamorphs have been described from Musa. However, very little is known about these taxa, and for the majority of these species no culture or DNA is available for study. In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set. The phylogeny inferred from the ITS region and the combined data set containing partial gene sequences of the actin gene, the small subunit mitochondrial ribosomal DNA and the histone H3 gene revealed a rich diversity of Mycosphaerella species on Musa. Integration of morphological and molecular data sets confirmed more than 20 species of Mycosphaerella (incl. anamorphs) to occur on banana. This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host. Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.
138 citations
TL;DR: Phylogenic analysis based on sequences of the internal transcribed spacer and 5.8S ribosomal DNA regions from the different leaf spot pathogens of bananas was consistent with the definition of a new species.
Abstract: A previously undescribed leaf spot disease of banana has been discovered in southern and Southeast Asia. The fungus identified as the causal agent of this leaf spot has a Mycosphaerella teleomorph stage and a Septoria anamorph stage. Isolation and reinoculation of the fungus to banana reproduced symptoms and confirmed its pathogenicity. Phylogenic analysis based on sequences of the internal transcribed spacer and 5.8S ribosomal DNA regions from the different leaf spot pathogens of bananas was consistent with the definition of a new species. M. eumusae (anamorph S. eumusae) is the name proposed for the causal agent and Septoria leaf spot as the name for the disease. The presence of the pathogen has been confirmed in leaf specimens from southern India, Sri Lanka, Thailand, Malaysia, Vietnam, Mauritius, and Nigeria.
79 citations
TL;DR: Leaf chlorosis and necrosis were often observed in advance of saprophytic growth of the mycelium on necrotic tissue, which supports previous reports suggesting secretion of a phytotoxin.
Abstract: A genetic transformation system has been developed for three Mycosphaerella pathogens of banana and plantain (Musa spp.). Mycosphaerella fijiensis and Mycosphaerella musicola, the causal agents of black and yellow Sigatoka, respectively, and Mycosphaerella eumusae, which causes Septoria leaf spot of banana, were transformed with a construct carrying a synthetic gene encoding green fluorescent protein (GFP). Most single-spored transformants that expressed GFP constitutively were mitotically stable in the absence of selection for hygromycin B resistance. Transformants of all three species were pathogenic on the susceptible banana cultivar Grand Nain, and growth in planta was comparable to wild-type strains. GFP expression by transformants allowed us to observe extensive fungal growth within leaf tissue that eventually turned necrotic, at which point the fungi grew saprophytically on the dead tissue. Leaf chlorosis and necrosis were often observed in advance of saprophytic growth of the mycelium on necrotic tissue, which supports previous reports suggesting secretion of a phytotoxin.
60 citations
Journal Article•
TL;DR: The teleomorph name, #Mycosphaerella eumusae#, and its anamorph, #Pseudocercospora euusae #, are validated for the banana disease formerly known as Septoria leaf spot, and can be distinguished based upon the morphology of their anamorphs.
Abstract: The teleomorph name, #Mycosphaerella eumusae#, and its anamorph, #Pseudocercospora eumusae#, are validated for the banana disease formerly known as Septoria leaf spot. This disease has been found on different #Musa# cultivars from tropical countries such as southern India, Sri Lanka, Thailand, Malaysia, Vietnam, Mauritius and Nigeria. It is contrasted with two similar species, namely #Mycosphaerella fijiensis# (black leaf streak or black Sigatoka disease) and #Mycosphaerella musicola# (Sigatoka disease). Although the teleomorphs of these three species are morphologically similar, they are phylogenetically distinct and can also be distinguished based upon the morphology of their anamorphs. (Resume d'auteur)
45 citations
TL;DR: It was revealed that more number of chitinase isoforms (CIs) were responses upon eumusa leaf spot stress than nematode stress.
Abstract: Knowledge on structure and conserved domain of Musa chitinase isoforms and their responses to various biotic stresses will give a lead to select the suitable chitinase isoform for developing biotic stress-resistant genotypes Hence, in this study, chitinase sequences available in the Musa genome hub were analyzed for their gene structure, conserved domain, as well as intron and exon regions To identify the Musa chitinase isoforms involved in Pratylenchus coffeae (root lesion nematode) and Mycosphaerella eumusae (eumusa leaf spot) resistant mechanisms, differential gene expression analysis was carried out in P coffeae- and M eumusae-challenged resistant and susceptible banana genotypes This study revealed that more number of chitinase isoforms (CIs) were responses upon eumusa leaf spot stress than nematode stress The nematode challenge studies revealed that class II chitinase (GSMUA_Achr9G16770_001) was significantly overexpressed with 675-fold (with high fragments per kilobase of exon per million fragments mapped (FPKM)) in resistant genotype (Karthobiumtham-ABB) than susceptible (Nendran-AAB) genotype, whereas when M eumusae was challenge inoculated, two class III CIs (GSMUA_Achr9G25580_001 and GSMUA_Achr8G27880_001) were overexpressed in resistant genotype (Manoranjitham-AAA) than the susceptible genotype (Grand Naine-AAA) However, none of the CIs were found to be commonly overexpressed under both stress conditions This study reiterated that the chitinase genes are responding differently to different biotic stresses in their respective resistant genotypes
21 citations
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| Year | Papers |
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| 2021 | 1 |
| 2019 | 2 |
| 2016 | 1 |
| 2015 | 1 |
| 2014 | 2 |
| 2013 | 1 |