Topic
Muri
About: Muri is a research topic. Over the lifetime, 21 publications have been published within this topic receiving 470 citations.
Papers
TL;DR: Experiments of insertional mutagenesis of the murI gene are reported which demonstrate that this gene is essential for the biosynthesis of D-glutamic acid, one of the specific components of cell wall peptidoglycan.
Abstract: The murI gene of Escherichia coli was recently identified on the basis of its ability to complement the only mutant requiring D-glutamic acid for growth that had been described to date: strain WM335 of E. coli B/r (P. Doublet, J. van Heijenoort, and D. Mengin-Lecreulx, J. Bacteriol. 174:5772-5779, 1992). We report experiments of insertional mutagenesis of the murI gene which demonstrate that this gene is essential for the biosynthesis of D-glutamic acid, one of the specific components of cell wall peptidoglycan. A special strategy was used for the construction of strains with a disrupted copy of murI, because of a limited capability of E. coli strains grown in rich medium to internalize D-glutamic acid. The murI gene product was overproduced and identified as a glutamate racemase activity. UDP-N-acetylmuramoyl-L-alanine (UDP-MurNAc-L-Ala), which is the nucleotide substrate of the D-glutamic-acid-adding enzyme (the murD gene product) catalyzing the subsequent step in the pathway for peptidoglycan synthesis, appears to be an effector of the racemase activity.
113 citations
TL;DR: The most important production concepts that are integral to Toyota production system theory and practice actually impede "leanness" as discussed by the authors, such as heijunka, or levelled production, and muri, or waste from overstressing machines and personnel.
Abstract: Advocates and critics alike have accepted 'lean' images of the Toyota production system. But certain production concepts that are integral to Toyota production system theory and practice actually impede 'leanness'. The most important of these are the concepts of heijunka, or levelled ('balanced') production, and muri, or waste from overstressing machines and personnel. Actual Toyota production systems exist as a compromise between these concepts and the pursuit of leanness via kaizen. The compromise between these contrasting tendencies is influenced by the ability of unions and other aspects of industrial relations regulation to counter practices such as shortnotice overtime and 'management by stress'.
71 citations
TL;DR: An SAR study of an HTS screening hit generated a series of pyridodiazepine amines as potent inhibitors of Helicobacter pylori glutamate racemase (MurI) showing highly selective anti-H.pylori activity, marked improved solubility, and reduced plasma protein binding.
50 citations
TL;DR: A successful scaffold-hopping approach gave a novel series of inhibitors of bacterial glutamate racemase (MurI) using 8-benzyl pteridine-6,7-diones as a source of inhibition and antibacterial activity.
38 citations
TL;DR: Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests that presence of a threshold concentration for theD- glutamate required by E.E. coli cells is suggested.
Abstract: D-Glutamate, an indispensable component of peptidoglycans of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests the presence of a threshold concentration for the D-glutamate required by E. coli cells, as well as a regulation system for murI expression.
21 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2016 | 2 |
| 2015 | 2 |
| 2014 | 1 |
| 2013 | 1 |
| 2009 | 2 |
| 2008 | 3 |