Monochamus nitens

Abstract: An adult male of Monochamus nitens (Bates) was collected from a dead Abies mariesii (Masters) tree on 5 July 2013 on Mount Fuji, Yamanashi, Japan (35°22′52.3′′N, 138°40′56.8′′E, 1750 m a.s.l.). The insect was dissected under a stereomicroscope (S8Apo, Leica Japan, Tokyo). Two species of nematodes, an aphelenchid and a diplogastrid, were isolated from the beetle. The aphelenchid and diplogastrid dauers were transferred to PDA that had been previously inoculated with Botrytis cinerea and to NGM that had been previously seeded with Escherichia coli OP50 strain, respectively. The media were incubated at 20°C and were occasionally observed to examine nematode propagation. Successfully propagated nematodes were subcultured to PDA-B. cinerea or NGM-OP50. The aphelenchid and diplogastrid were morphologically identified as Bursaphelenchus mucronatus Mamiya & Enda, 1979 and an undescribed Diplogasteroides sp., respectively. Detailed morphological and phylogenetic analyses and a formal taxonomic identification and description of Diplogasteroides sp. will be presented elsewhere; the isolation record and molecular profiles of B. mucronatus are provided here. The general morphology of nematodes from a 2-week-old culture was examined using methods described elsewhere (Kanzaki, 2013). The specimens were confirmed as B. mucronatus kolymensis Korentchencko, 1980 (Braasch, Gu & Burgermeister, 2011) based on the sub-cylindrical female tail with a clearly offset mucron (Mamiya & Enda, 1979; Korentchenko, 1980; Braasch et al., 2011). The molecular profiles of nematodes from the same culture were also examined, i.e., near full length of the