Topic
Microtechnology
About: Microtechnology is a research topic. Over the lifetime, 197 publications have been published within this topic receiving 7087 citations.
Papers published on a yearly basis
Papers
TL;DR: This work presents a miniaturized, multiwell culture system for human liver cells with optimized microscale architecture that maintains phenotypic functions for several weeks and demonstrates utility through assessment of gene expression profiles, phase I/II metabolism, canalicular transport, secretion of liver-specific products and susceptibility to hepatotoxins.
Abstract: Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-driven microtechnology in the biomedical arena now allows fabrication of microscale tissue subunits that may be functionally improved and have the advantages of miniaturization. Here we present a miniaturized, multiwell culture system for human liver cells with optimized microscale architecture that maintains phenotypic functions for several weeks. The need for such models is underscored by the high rate of pre-launch and post-market attrition of pharmaceuticals due to liver toxicity. We demonstrate utility through assessment of gene expression profiles, phase I/II metabolism, canalicular transport, secretion of liver-specific products and susceptibility to hepatotoxins. The combination of microtechnology and tissue engineering may enable development of integrated tissue models in the so-called 'human on a chip'.
1,242 citations
TL;DR: This tutorial review outlines the fundamental work performed on continuous flow acoustic standing wave separation of particles in macro scale systems and discusses several potential applications in life science research and in the medical clinic.
Abstract: Acoustic standing wave technology combined with microtechnology opens up new areas for the development of advanced particle and cell separating microfluidic systems. This tutorial review outlines the fundamental work performed on continuous flow acoustic standing wave separation of particles in macro scale systems. The transition to the microchip format is further surveyed, where both fabrication and design issues are discussed. The acoustic technology offers attractive features, such as reasonable throughput and ability to separate particles in a size domain of about tenths of micrometers to tens of micrometers. Examples of different particle separation modes enabled in microfluidic chips, utilizing standing wave technology, are described along a discussion of several potential applications in life science research and in the medical clinic. Chip integrated acoustic standing wave separation technology is still in its infancy and it can be anticipated that new laboratory standards very well may emerge from the current research.
902 citations
655 citations
TL;DR: The selective and real-time detection of label-free DNA using an electronic readout demonstrates the most direct and simple translation of genetic information to microelectronics.
Abstract: We report the selective and real-time detection of label-free DNA using an electronic readout. Microfabricated silicon field-effect sensors were used to directly monitor the increase in surface charge when DNA hybridizes on the sensor surface. The electrostatic immobilization of probe DNA on a positively charged poly-l-lysine layer allows hybridization at low ionic strength where field-effect sensing is most sensitive. Nanomolar DNA concentrations can be detected within minutes, and a single base mismatch within 12-mer oligonucleotides can be distinguished by using a differential detection technique with two sensors in parallel. The sensors were fabricated by standard silicon microtechnology and show promise for future electronic DNA arrays and rapid characterization of nucleic acid samples. This approach demonstrates the most direct and simple translation of genetic information to microelectronics.
584 citations
Book•
1 Jan 1998
TL;DR: Laser assisted microtechnology deals with laser applications to a wide variety of problems in microelectronic design and fabrication as mentioned in this paper, covering micromachining of thin films, microprocessing of materials, maskless laser micropatterning and laser-assisted synthesis of thin systems.
Abstract: Laser-Assisted Microtechnology deals with laser applications to a wide variety of problems in microelectronic design and fabrication It covers micromachining of thin films, microprocessing of materials, maskless laser micropatterning and laser-assisted synthesis of thin systems The monograph describes fundamental aspects and practical details of the technological processes as well as the optimum conditions for their realization
522 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 5 |
| 2024 | 6 |
| 2023 | 9 |
| 2022 | 12 |
| 2021 | 2 |
| 2020 | 3 |