TL;DR: Pestalotiopsis microspora was isolated from the inner bark of a small limb of Himalayan yew, and was shown to produce taxol in mycelial culture, and spectroscopic and chromatographic comparisons with authentic taxol were identified.
Abstract: Summary: Pestalotiopsis microspora was isolated from the inner bark of a small limb of Himalayan yew, Taxus wallachiana, and was shown to produce taxol in mycelial culture. Taxol was identified by spectroscopic and chromatographic comparisons with authentic taxol. Optimal taxol production occurred after 2-3 weeks in still culture at 23°C. [14C]Acetate and [14C]phenylalanine served as precursors for fungal [14C]taxol. These observations on P. microspora are discussed in relation to the biological importance of taxol production by fungi in general.
TL;DR: This study examined the ability of Microspora and Lemna minor to remove soluble lead and nickel under various laboratory conditions and found no synergistic/antagonistic effect was noted for the multiple metal experiments, in terms of metal removal.
TL;DR: In the case of one strain of P. microspora (CP-4), taxol was isolated from culture medium and was shown to be identical to authentic taxol by chromatographic and spectroscopic means.
Abstract: Pestalotiopsis microspora occurs as a range of strains in bald cypress, Taxodium distichum. The organisms live as endophytes in the bark, phloem and xylem, and isolates show differences in cultural and microscopic characteristics on common laboratory media. Many of these fungi make taxol as determined by the reactivity of partially purified culture extracts with specific monoclonal antibodies against taxol. In the case of one strain of P. microspora (CP-4), taxol was isolated from culture medium and was shown to be identical to authentic taxol by chromatographic and spectroscopic means.
TL;DR: An examination of the endophytic fungal communities of wild torreyas consistently found a filamentous fungus, Pestalotiopsis microspora, associated with diseased trees and also with most symptomless trees, and which produces the phytotoxins pestalopyrone, hydroxypestalopyrsone, and pestaloside which give rise to the disease.
TL;DR: Consumption was significantly correlated with the nutrient content of food items, whereas G/I% and growth rate were only weakly related to food quality.
Abstract: 1. We investigated the growth of the detritivore-shredder Sericostoma personatum by feeding groups of larvae on a fresh macrophyte, Potamogeton perfoliatus, conditioned sitka spruce needles, Picea sitchensis, conditioned alder leaves, Alnus glutinosa, conditioned ash leaves, Fraxinus excelsior, and a fresh filamentous green alga, Microspora sp. A sixth treatment group of larvae was fasted.
2. The nitrogen content of the food items ranged from 1.4% dry weight (DW) for Picea needles to 4.4% DW for Microspora filaments. Consumption of the various food items by Sericostoma differed significantly, being highest for Picea, and lowest for Potamogeton and Microspora.
3. The instantaneous growth rate ranged from −1.3% DW day−1 in the fasting group to 0.75% DW day−1 in the Alnus group. The growth rate of larvae fed on Alnus, Fraxinus and Microspora was similar, and significantly higher than that of the other three groups. An increase in the size of larval cases followed the same pattern as larval growth, although with less variation between food items.
4. Larvae fed on Microspora exhibited the greatest increase in fat content, while that of the larvae fed on Picea and those in the fasting group decreased. The gross growth efficiency (G/I%) of Sericostoma (larva + case) ranged from 2% when fed on Picea to 34% when fed on Microspora. Consumption was significantly correlated with the nutrient content of food items, whereas G/I% and growth rate were only weakly related to food quality.
5. The growth of Sericostoma varies with diet and the food items sustaining highest growth rate are not necessarily the ‘typical’ shredder food resources.