Topic
Microscopy and Microanalysis
About: Microscopy and Microanalysis is a research topic. Over the lifetime, 82 publications have been published within this topic receiving 1249 citations.
Papers published on a yearly basis
Papers
Journal Article•
TL;DR: The design and fabrication of a three-dimensional in vitro system to model vascular stenosis so that specific cellular interactions and responses to hemodynamic stimuli can be investigated and serve as an in vitro 3D culture system to investigate vascular pathogenesis.
Abstract: Vascular stenosis triggers adaptive cellular responses that induce adverse remodeling, which can progress to partial or complete vessel occlusion. Despite its severity, cellular interactions and biophysical cues that regulate pathological progression are poorly understood. We report the design and fabrication of a three-dimensional in vitro system to model vascular stenosis so that specific cellular interactions and responses to hemodynamic stimuli can be investigated. Tubular cellularized constructs (cytotubes) were produced using a collagen casting system to generate a stenotic arterial model. Fabrication methods were developed to create cytotubes containing co-cultured vascular cells, where cell viability, distribution, morphology, and contraction were examined (Figure). Fibroblasts, bone marrow primary cells, smooth muscle cells (SMCs), and endothelial cells (ECs) remained viable during culture and developed locationand time-dependent morphologies. We found cytotube contraction to depend on cellular composition, where SMC-EC co-cultures adopted intermediate contractile phenotypes between SMCand EC-only cytotubes. Our fabrication approach and resulting artery model can serve as an in vitro 3D culture system to investigate vascular pathogenesis.
787 citations
TL;DR: In this article, fly ash-slag inorganic polymer cement (i.e., "fly ash geopolymer" binders) was studied and a more detailed understanding of the gel structure and its formation mechanism was developed.
Abstract: By scanning electron microscopy and microanalysis of fly ash-based and mixed fly ash-slag inorganic polymer cement (i.e., “fly ash geopolymer”) binders, a more detailed understanding of the gel structure and its formation mechanism have been developed. The binder is predominantly an aluminosilicate gel charge balanced by alkali metal cations, although it appears that calcium supplied by slag particles becomes relatively well dispersed throughout the gel. The gel itself is comprised of colloidal-sized, globular units closely bonded together at their surfaces. The microstructure of the binder resulting from hydroxide activation of fly ash is much less uniform than that which forms in a corresponding silicate-activated system; this can be rationalized in terms of a newly developed explanation for the differences in reaction mechanisms between these two systems. In hydroxide activation, the newly formed gel phase nucleates and grows outwards from the ash particle surfaces, whereas the high silica concentration in a silicate-activated system enables a more homogeneous gelation process to take place throughout the inter-particle volume.
168 citations
Book•
1 Feb 2002
TL;DR: High resolution microscopy and microanalysis self-organized and quantum domain structures epitaxy - growth phenomena epitaxy, wind band gap nitrides processed silicon, substrates and dielectrics metalization silicides amd contacts device studies and specimen preparation scanning probe microscopy advanced scanning electron and optical microscopy.
Abstract: High resolution microscopy and microanalysis self-organized and quantum domain structures epitaxy - growth phenomena epitaxy - wind band-gap nitrides processed silicon, substrates and dielectrics metalization silicides amd contacts device studies and specimen preparation scanning probe microscopy advanced scanning electron and optical microscopy.
120 citations
TL;DR: In this article, the authors review progress in the characterization of polyamide separation membranes using transmission electron microscopy, atomic force microscopy and vibrational spectroscopy, positron annihilation, nuclear magnetic resonance, and Rutherford backscattering spectrometry.
Abstract: The polyamide active layers of commercial reverse-osmosis and nanofiltration membranes are examples of nanoscale functional materials that challenge the state of the art of materials characterization. The active layer is only ∼100 nm thick, and because the active layer is formed by a process of interfacial polymerization, the structure and composition of the membrane is highly inhomogeneous. Even such basic physical and chemical properties of the membrane as the atomic density, swelling in water, distribution of charged species, and the mobility of water and ions, are poorly understood. In this article, we briefly review progress in the characterization of polyamide separation membranes using transmission electron microscopy, atomic force microscopy, vibrational spectroscopy, positron annihilation, nuclear magnetic resonance, and Rutherford backscattering spectrometry. Advances in the microanalysis methods applicable to these complex materials will advance fundamental understanding of the structure–property relationships of polymer membranes and further the long-term goal of synthesizing membranes with improved performance.
104 citations
100 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2018 | 3 |
| 2017 | 7 |
| 2016 | 2 |
| 2015 | 3 |
| 2014 | 5 |