Topic
Microbial Solid Culture
About: Microbial Solid Culture is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 1 citations.
Papers
Patent•
2 Apr 2014
TL;DR: In this article, a method for performing microbial solid culture by using a microbial solid-culture device consisting of a box body, a first inlet pipe, an outlet pipe and a sieve plate with sieve pores was described.
Abstract: The invention discloses a method for performing microbial solid culture by using a microbial solid culture device. The device comprises a box body, a first inlet pipe, an outlet pipe and a sieve plate with sieve pores, wherein the sieve plate is arranged in the box body and separates the inner space of the box body into an upper layer and a lower layer; the first inlet pipe is communicated with the lower layer of the box body; the box body is communicated with the outside through the outlet pipe. The method comprises the following steps: introducing steam to sterilize a material in the box body, inoculating a microbial fermentation seed to be cultured into the box body, enabling the microbial fermentation seed to be in contact with the material, and culturing; in the culturing process, introducing sterile gas into the box body, wherein the introduced steam or sterile gas enters the upper layer of the box body through the first inlet pipe and the sieve pores in the sieve plate to be in contact with the material or the material inoculated with the fermentation seed, the gas subjected to contact is discharged from the box body through the outlet pipe, and the material can not pass through the sieve pores. According to the invention, the microbial solid culture efficiency is high.
1 citations
Patent•
16 Jan 2013
TL;DR: In this article, a sterile grinding method for a microbial solid culture medium has been proposed, which has the advantages of freeness of pollution, small damages to microorganisms during grinding, high culture medium utilization rate and the like, can choose different time, strength and moving tracks according to needs, and can meet requirements on solid culture of various microorganisms.
Abstract: The invention relates to a sterile grinding method for a microbial solid culture medium. The method includes following steps: applying force to a container containing the microbial solid culture medium to enable the container to reciprocate according to a preset track and enable the solid culture medium in the container to form reciprocating motion material flow opposite to the moving track of the container. The grinding method for the microbial solid culture medium has the advantages of freeness of pollution, small damages to microorganisms during grinding, high culture medium utilization rate and the like, can choose different time, strength and moving tracks according to needs, and can meet requirements on solid culture of various microorganisms. The invention further discloses a cultivation method for the microbial solid culture medium. During cultivation, the solid culture medium is grinded by intermittently adopting the grinding method, and compared with conventional solid culture technologies, the cultivation method for the microbial solid culture medium can remarkably improve microbial culture quality and efficiency and is suitable for large-scale application in fields of biopharmacy, food fermentation, biochemical test and the like.
Patent•
4 Jan 2017
TL;DR: In this paper, a comprehensive utilization method of amino acid wastewater is proposed, which includes the following steps: selecting solid culture medium raw materials according to the nutrient demands of target microorganisms to be cultured, grinding the raw materials and then uniformly mixing according to a ratio to form a matrix, adding amino acids wastewater, adjusting the pH value to a range required by the target micro organisms to be grown after placing for 6 to 12 hours, then adding sterile nutrient solutions to adjust the ratio of carbon to nitrogen according to their requirements.
Abstract: The invention discloses a comprehensive utilization method of amino acid wastewater The method specifically comprises the following steps: selecting solid culture medium raw materials according to the nutrient demands of target microorganisms to be cultured, grinding the raw materials and then uniformly mixing according to the ratio to form a matrix, adding amino acid wastewater, adjusting the pH value to a range required by the target microorganisms to be cultured after placing for 6 to 12 hours, then adding sterile nutrient solutions to adjust the ratio of carbon to nitrogen according to the requirement of the ratio of carbon to nitrogen required by the growth of the target microorganisms to be cultured, thus forming a special solid culture medium of which nutritional components and the target microorganisms are in compatibility; the comprehensive utilization method of amino acid wastewater provided by the invention is simple and convenient and fast, provides a new way for sterilization of the microbial solid culture medium while effectively absorbing amino acid wastewater, has the same or similar sterilization effect with the existing method and realizes recycling and high-efficient utilization of waste and the coordinated development of environmental protection, thereby having great economic, environmental, social and ecological benefits
Performance Metrics
| Year | Papers |
|---|---|
| 2017 | 1 |
| 2014 | 1 |
| 2013 | 1 |