About: Microbial Concentration Method is a research topic. Over the lifetime, 2 publications have been published within this topic receiving 2 citations.
TL;DR: In this paper, a method to achieve approximately 4-8 times the microbial concentration by using diatomite when compared with an existing prior, and a method was developed that can directly extract nucleic acids from concentrated microorganisms by using dimethyl adipimidate (DMA) or the like.
Abstract: The present invention relates to a microbial concentration method using diatomite and a nucleic acid extraction method. A technique was developed that can achieve approximately 4-8 times the microbial concentration by using diatomite when compared with an existing prior, and a method was developed that can directly extracts nucleic acids from concentrated microorganisms by using dimethyl adipimidate (DMA) or the like. The microbial concentration and nucleic acid extraction techniques allowable in a single tube are expected to significantly reduce contamination coming from the outside, costs, time, complexity, and the like.
TL;DR: In this paper, a method of concentrating microorganisms and extracting nucleic acids using diatomaceous earth was proposed, which is expected to significantly reduce external contamination, cost, time and complexity by enabling microbial concentration and nucleic acid extraction techniques in one tube.
Abstract: The present invention relates to a method of concentrating microorganisms and a method of extracting nucleic acids using diatomaceous earth and developed a technology capable of concentrating microorganisms about 4-8 times using the modified diatomaceous earth and directly extracting nucleic acids from the concentrated microorganism using dimethyl adipimidate (DMA). It is expected to significantly reduce external contamination, cost, time and complexity by enabling microbial concentration and nucleic acid extraction techniques in one tube.