Topic
Microbacterium mitrae
About: Microbacterium mitrae is a research topic. Over the lifetime, 2 publications have been published within this topic receiving 9 citations.
Papers
TL;DR: Two strains of Gram-stain-positive, aerobic,Non-spore-forming, non-motile, rod-shaped bacteria isolated from the faeces of Tibetan gazelle collected from the Qinghai-Tibet Plateau, PR China represent a novel species of the genus Microbacterium.
Abstract: Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China. The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively). The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium , and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T. The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl. The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively. In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids. The major cell-wall sugars were rhamnose, ribose and galactose. Alanine, glutamic acid, glycine and ornithine were in the cell-wall peptidoglycan. Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium , named here as Microbacterium wangchenii sp. nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
TL;DR: Data obtained from DNA-DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain M4-8(T) represents a novel species within the genus Microbacterium.
Abstract: A novel bacterium (strain M4-8T) belonging to the genus Microbacterium was isolated from salted turban shell, which is a traditional fermented food in Korea Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized Cells of this strain were Gram-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies It grew in 0–8 % (w/v) NaCl and at 15–37 °C, with optimal growth occurring in 1 % (w/v) NaCl and at 30 °C Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M4-8T is associated with members of the genus Microbacterium Within the phylogenetic tree, this novel strain shared a branching point with Microbacterium hominis IFO 15708T (978 % similarity) The DNA G+C content was 713 mol% and DNA–DNA hybridization experiments showed a low level (<29 %) of DNA–DNA relatedness between M4-8T and its closest relatives The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0 and the major cell-wall diamino acid was ornithine Data obtained from DNA–DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain M4-8T represents a novel species within the genus Microbacterium The name Microbacterium mitrae sp nov is proposed, with M4-8T (=KACC 21129T =JCM 16363T) as the type strain
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Performance Metrics
| Year | Papers |
|---|---|
| 2020 | 1 |
| 2011 | 1 |